甘氨双唑钠对乏氧胰腺癌细胞放射增敏研究  

Radiosensitization effect of CMNa on hypoxic pancreatic cancer cell in vitro

作　　者：尹立杰[1] 张力[2] 丁田贵[1] 彭兆祥[1] 于环[2] 高玉伟[1] 

机构地区：[1]大连大学医学院附属大连中心医院放疗科,116033 [2]大连大学医学院附属大连中心医院实验室,116033

出　　处：《中华放射医学与防护杂志》2006年第5期467-470,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究甘氨双唑钠(CMNa)在乏氧环境下对人胰腺癌Panc-1细胞的放射增敏效果。方法在乏氧和加不同浓度CMNa环境下对体外培养的人胰腺癌Panc-1细胞进行单次大剂量的γ射线照射,通过微孔板荧光酶标仪检测细胞死亡率来研究放射增敏作用。CMNa在乏氧环境下的选择性增敏效果利用流式细胞技术进行验证。结果胰腺癌Panc-1细胞30Gy照射后的细胞死亡率在乏氧环境下随CMNa浓度的增加显著增高;而在常规环境下CMNa却无明显的放射增敏作用。CMNa在乏氧环境下的选择性增敏作用具有时间相关性。结论CMNa在乏氧环境下对人胰腺癌细胞大剂量γ射线照射具有明显的增敏效果。Objective To investigate the effects of glycodidazolum natrium（CMNa） on pancreatic cancer cells under hypoxic condition. Methods The human pancreatic cancer Panc-1 cells were exposed to a single fraction of high-dose T-ray radiation either with CMNa or under hypoxic condition. The percentage of dead cells was detected with a muhiwell plated reader, and fluorescence intensities of propidium iodide were measured before and after digitonin treatment. The sensitizing effect of CMNa on cell killing induced by high-dose irradiation was evaluated by time and concentration dependence. The selective radioscnsitive effect of CMNa on hypoxia was evaluated by flow cytometry. Results The death rate of pancreatic cancer Panc-1 cells paralleled with the increasing concentration of CMNa under hypoxie condition after 30 gray irradiation. The selective radioscnsitive effect of CMNa on hypoxia was time-dependent. Conclusions CMNa can enhance the radioscnsitivity of pancreatic cancer Panc-1 cells under hypoxic condition with high-dose irradiation.

关 键 词：胰腺癌细胞 放射增敏 细胞乏氧 甘氨双唑钠 

分 类 号：R735.9[医药卫生—肿瘤]