肿瘤细胞对γ-干扰素的敏感性影响其在体环境中的致瘤性  

The sensitivity of tumor cells to interferon-γ and tumorigenicity in vivo

作　　者：苏伟[1] 黄美雄[1] 韦军民[1] 

机构地区：[1]卫生部北京医院普外科,北京100730

出　　处：《中华实验外科杂志》2006年第11期1389-1390,共2页Chinese Journal of Experimental Surgery

摘　　要：目的利用从Panc-02细胞株分离出的两种对γ-干扰素(IFN-γ)敏感性不同的肿瘤细胞,探讨肿瘤细胞IFN-γ敏感性对其在体试验中致瘤性的影响。方法IFN-γ刺激Panc-02细胞后,根据Ⅰ型表面组织相容抗原(MHCⅠ)的表达,利用流式细胞分类技术,分离出FS-3和PM-9细胞。将这些肿瘤细胞注射于小鼠的皮下,在21 d里比较肿瘤的生长情况。结果IFN-γ刺激FS-3,PM-9和Panc-02细胞后,FS-3的MHCⅠ表达最高,对IFN-γ的敏感性高；PM-9的MHCⅠ表达最低,对IFN-γ的敏感性低。把这3种肿瘤细胞注射于小鼠的皮下后,PM-9肿瘤的生长明显快于FS-3和Panc-02肿瘤。21 d后PM-9肿瘤的大小(139．1±30．2)mm^2明显大于FS-3和Panc-02肿瘤[(0．8±1．6),(14．3±6．7)mm^2,P＜0．01]。注射PM-9细胞的小鼠全部形成肿瘤(n=24,100%),而注射FS-3或Panc-2细胞的小鼠只有部分形成肿瘤(n=12,30%；n=24,75%；P＜0．05)。结论肿瘤细胞对IFN-γ敏感性影响其在体环境中的致瘤性。Objective The sensitivity of tumor cells to interferon-y （IFN-y） was investigated using two kinds of tumor cells separated from Panc-02 cells, whose sensitivities to IFN-γ were different. Methods After Panc-02 cells were stimulated with IFN-γ,FS-3 and PM-9 cells were separated based on the expression of MHC I by flow cytometry cell-sorting techniques. These tumor cells were injected subcutaneously in mice. The sizes of subcutaneous tumors were monitored in 21 days. Results After FS-3, Panc-02 and PM-9 cells were stimulated with IFN-γ, the expression of MHC I was different. FS-3 cells were sensitive to IFN-γ and PM-9 cells insensitive to IFN-γ. After these tumor cells were injected subcutaneously in mice, the growth of PM-9 tumors was faster than that of FS-3 and Panc-02 tumors. After 21 days,the sizes of PM-9 tumors （139.1± 30.2） mm^2 were larger than those of FS-3 and Panc-02 tumors [ （0.8 ± 1.6）, （ 14.3 ± 6.7） mm^2, P〈 0.01 ]. On the 21st day, PM-9 tumors were observed in all of mice （n = 24,100 % ） ,FS-3 tumors and Panc-2 tumors were observed in parts of mice （n = 12.30 % and n = 24.75 % respectively; P 〈 0.05）. Conclusion The sensitivity of tumors to IFN-γ affects its tumorigenicity in in vivo experiment.

关 键 词：肿瘤细胞 Γ-干扰素 敏感性 致瘤性 

分 类 号：R73-3[医药卫生—肿瘤]