应用PCNA和survivin预测鼻咽癌放疗敏感性的研究  

A Study on PCNA and Survivin as the Molecule Marks to Predict Tumor Radiosensitivity of Nasopharyngeal Carcinoma

作　　者：黄东海[1] 田勇泉[1] 邱元正[1] 范松青[2] 李桂源[2] 

机构地区：[1]中南大学湘雅医院耳鼻咽喉科,湖南长沙410008 [2]中南大学肿瘤研究所,湖南长沙410011

出　　处：《同济大学学报（医学版）》2006年第5期39-42,共4页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金资助项目(30200312)

摘　　要：目的研究PCNA和survivin作为鼻咽癌放疗敏感性预测的候选分子标志。方法应用自制的组织微阵列,结合免疫组化技术,检测PCNA和survivin在鼻咽癌放疗敏感者和不敏感者中的表达情况,同时对其作为预测鼻咽癌放疗敏感性的分子指标进行有效性评价。结果PCNA在鼻咽癌放疗敏感者和放疗不敏感者阳性表达率分别为94.1%(177/188)和63.4%(26/41),两者比较有统计学差异(P<0.01);而survivin的阳性表达率分别为89.4%(169/189)和56.1%(18/41),两者比较有统计学差异(P<0.01)。对PCNA作为预测鼻咽癌放疗敏感性分子指标进行有效性评价,结果其灵敏度为94.1%,特异度为31.7%,Youden指数为0.31,符合率为83.8%,阳性预测值和阴性预测值分别为87.2%和57.7%;而survivin的灵敏度为89.4%,特异度为43.9%,Youden指数为0.33,符合率为81.3%,阳性预测值和阴性预测值分别为88.2%和47.4%。结论PCNA和survivin可作为预测鼻咽癌放疗敏感性正相关的候选分子指标之一。Objective To study PCNA and survivin as the molecule marks to predict the tumor radiosensitivity of nasopharyngeal carcinoma. Methods With our stir-made tissue microarrays and immunohistochemistry methods to study the expression of PCNA and survivin in radio-resistant group and radio-sensitive group of nasopharyngeal carcinoma. Restilts The positive expression rates of PCNA in radio-resistant group and radiosensitive group were 94.1% （177/188） and 63.4 % （26/41） （P 〈 0.01）. The positive expression rates of survivin were 89.4 % （ 169/189） and 56.1% （18/41） （P〈0.01）. The sensitivity, specificity, Youden index, accuracy, positive predictive value and negative predictive value of PCNA for tumor radio-sensitivity were 94.1% ,31.7%, 0.31, 83.8%, 87.2% and 57.7% respectivdy. Those of survivin were 89.4% ,43.9% ,0.33,81.3% ,88.2% and 47.4% .Conclusion The PCNA and survivin may be one of the positive candidate molecule marks to predict the tumor radiosensitivity of nasopharyngeal carcinoma.

关 键 词：PCNA SURVIVIN 鼻咽肿瘤 肿瘤放疗敏感性 

分 类 号：R739.6[医药卫生—肿瘤]