猪恶性高温综合征（MHS）基因群体检测及部分片段DNA序列研究  

The Population Investigations and DNA Sequence Study of Porcine MHS Gene

作　　者：李来记[1] 张沅[1] 李宁[1] 

机构地区：[1]中国农业大学动物科技学院

出　　处：《中国兽医学报》1996年第6期540-545,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金

摘　　要：本研究主要包括：（１）建立了猪ＭＨＳ基因检测的ＰＣＲ－ＲＦＬＰ方法。该方法具有准确、快速和无侵害的特点；（２）利用猪ＭＨＳ基因的ＰＣＲ－ＲＦＬＰ方法对中国现有猪种２０个群体的ＭＨＳ基因座位的基因和基因型频率进行了检测。结果表明，属于中国的本地品种五指山猪、香猪，引进品种约克夏猪，均不含有ＭＨＳ基因；在中国本地品种二花脸猪和民猪中，发现存在ＭＨＳ基因，且民猪的频率较高（０．１５６２）；培育品种北京花猪，引进品种丹系长白猪和杜洛克猪，均有ＭＨＳ基因；引进品种皮特兰猪、比系长白猪，ＭＨＳ基因频率最高，分别为０．９５２０和０．９４３２；（３）猪ＲＹＲ１／ＣＲＣ基因部分ＤＮＡ序列品种间比较，ＭＨＳＮＮ香猪和二花脸猪的１８４３位为“Ｃ”，而ＭＨＳｎｎ为“Ｔ”；This study consists of three parts: (1) A PCR RFLP test was established to identify the gene at MHS locus. The DNA base test of MHS gene is a rapid, accurate and uninvasive method. (2) The gene and genotype frequencies of MHS locus in 20 pig populations in China were investigated by the PCR RFLP test. MHS gene exists in Erhualian and Min Pig of China native breed; Beijing Hua Pig of China developed breed; Pietrain, Duroc, Belgian Landrace and Danish Landrace of China exotic breed; Line A, B and C of Dekalb Pig, being not found in Wuzisan,Xiangzhu,Yorkshire,Beijing Heizhu and Dekalb E,F lines. In MHS gene frequency, the highest populations are Pietrain (0.951 9) and Belgian Landrace (0.943 2); the middle ones are Min Pig (0.156 2), Line B (0.171 6) and C (0.084 6) of Dekalb; the lower ones are Duroc (0.04), Danish Landrace (0.035 7) and Beijing Hua Pig (0.036 5); and the lowest is Erhualian Pig (0.005 7). (3) Comparison among the DNA sequences of different breeds. The T base is at the 1843 site of MHS gene of MHS nn Pietrain Pig, but C in the same site of MHS NN Xiang Pig and Erhualian Pig. In 1 795￣1 928 coding region, the some other base variations are also found.

关 键 词：恶性高温综合征 基因诊断 聚合酶链式反应 DNA 

分 类 号：S858.280.4[农业科学—临床兽医学]