低氧微环境对MDA-435细胞株MMP2、9及其抑制剂TIMP2、1表达的影响  

Effect of hypoxia on MMP2,9 and TIMP2,1 in human breast cancer cell line

作　　者：郭文菁[1] 曲迅[2] 张彬彬[2] 邵倩倩[2] 宋丙凤[2] 高文娟[2] 刘佳[2] 王春娥[2] 孔北华[2] 

机构地区：[1]山东大学第二医院妇产科,山东济南250033 [2]山东大学齐鲁医院临床基础研究所,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第10期978-980,984,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30472261)

摘　　要：目的:探讨低氧微环境对人乳腺癌细胞株MDA-435中基质金属蛋白酶(matrix metalloproteinases,MMPs)及其抑制剂(tissue inhibitors of matrix metalloproteinases,TIMPs)表达的影响。方法:分别置人乳腺癌细胞株MDA-435于常氧(21%O25、%CO2)和低氧(1%O25、%CO2和94%N2)环境中培养6 h,RT-PCR技术mRNA水平检测MMP2、9及TIMP2、1的表达。结果:MDA-435细胞株表达MMP9和TIMP1 mRNA,未检测到MMP2和TIMP2mRNA;低氧环境下MDA-435细胞株中MMP9 mRNA水平显著上升(P<0.05),TIMP1基因的表达在低氧环境与常氧环境下差异无统计学意义(P>0.05)。结论:与常氧环境相比,低氧环境下乳腺癌细胞株MDA-435中MMP9及其抑制剂TIMP1的表达平衡发生了改变,提示,进行肿瘤转移分子机制的体外研究应充分考虑氧环境的影响。Objective： To investigate the effect of hypoxia on the expressions of MMPs and TIMPs in human breast carcinoma cell line MDA-435. Methods： After the MDA-435 ceils being cultured under normoxia（21% 02, 5% CO2 ）or hypoxia（ 1% O2, 5% CO2 and 94% N2 ）for 6 hours, the expressions of MMP2, 9 and TIMP2, 1 were detected in mRNA level by RT-PCR. Results： The expressions of MMP2 and TIMP2 were not observed in human breast cancer cell line MDA-435 ; the expression of MMP9 increased significantly in mRNA levels when cells were cultured under normoxia （ P 〈 0.05）, but no significant differences of TIMPI were observed under normoxia or hypoxia （ P 〉 0.05 ）. Conclusion： Hypoxia could affect the expression of MMP9 in human breast cancer cell line MDA-435, indicating that in vitro study on the molecule mechanism of neoplasm metastasis should take the oxygen environment into conclusion.

关 键 词：乳腺肿瘤 低氧 基质金属蛋白酶 

分 类 号：R737.9[医药卫生—肿瘤]