M2-PK蛋白在宫颈及癌变组织中的差异表达  

Differential expression of M2-PK in normal cervix and cervical cancer tissues

作　　者：白晓卉[1] 孔丽 张爱荣[2] 贾继辉[1] 张魏芳[1] 唐伟[1] 于修平[1] 

机构地区：[1]山东大学医学院病原微生物学教研室,山东济南250012 [2]山东大学第二医院妇产科,山东济南250033

出　　处：《山东大学学报（医学版）》2006年第10期981-984,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30370064)

摘　　要：目的:筛选、验证肿瘤标志物M2-PK在宫颈及癌变组织中的差异表达。方法:选择永生化的人宫颈粘膜上皮细胞株H8和人宫颈癌细胞株Caski,采用双向凝胶电泳技术通过比较二者的双向电泳图谱筛选明显差异表达的蛋白质,并行MALDI-TOF-MS质谱鉴定;Western blot法检测差异蛋白质M2-PK在宫颈及癌变组织中的表达。结果:获得H8和Caski细胞的双向电泳图谱;蛋白M2-PK在Caski细胞中的表达量明显高于H8细胞;M2-PK在宫颈的正常组织、癌前病变组织和癌组织中的表达逐渐增加。结论:筛选出H8细胞和Caski细胞间的差异蛋白M2-PK,M2-PK在宫颈及癌变组织中的表达量不同。Objective： To screen out cervical carcinoma related protein markers by a comparative proteome analysis. Methods： Two-dimensional gel electrophoresis maps of the proteins extracted from H8 and Caski were applied respectively. The differential expression proteins were identified by MALDI-TOF-MS; Western blot was used to detect M2-PK expression from normal cervix and cervical carcinoma tissues. Results： The 2-DE maps of H8 cells and Caski cells were established, and the protein M2-PK was identified successfully. Western blot confimaed differential expression of M2- PK on normal cervix and cervical carcinoma tissues. Conclusion： These proteins from H8 cells and Caski cells are differentially expressed. It will be helpful to find new molecular markers for early diagnosis and treatment of cervical cancer.

关 键 词：蛋白质组 电泳 凝胶 双向 宫颈肿瘤 M2型丙酮酸激酶 蛋白印迹 

分 类 号：R737.33[医药卫生—肿瘤]