p38信号蛋白在乳腺浸润性导管癌中的表达及其与淋巴结转移的关系  

Expression of p38 mitogen-activated protein kinase and its relationship with lymph node metastasis in breast invasive ductal carcinoma

作　　者：李凤[1] 韩艳春[1] 曾宪旭[1] 关剑[1] 王睿[1] 宋敏[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,辽宁沈阳110001

出　　处：《中国医科大学学报》2006年第5期467-469,共3页Journal of China Medical University

基　　金：辽宁省教育厅科研基金资助项目(2004D107)

摘　　要：目的:探讨p38信号蛋白在乳腺浸润性导管癌中的表达及其与临床病理特征之间的关系。方法:应用免疫组织化学S-P法检测60例乳腺浸润性导管癌,Western blot方法检测30例乳腺浸润性导管癌及5例癌旁正常组织中p38、磷酸化p38(p-p38)的表达,分析p38、p-p38与淋巴结转移等临床病理特征之间的关系。结果:免疫组化结果显示,p38蛋白主要分布于乳腺癌上皮细胞与正常乳腺上皮细胞的胞浆内,癌细胞胞浆浓染,正常乳腺细胞胞浆淡染;p-p38蛋白阳性信号定位于乳腺癌细胞核。有淋巴结转移与无淋巴结转移的乳腺癌组织中p38的阳性率分别为76.3%和40.9%,差异十分显著(P<0.01);p-p38的阳性率分别为68.4%和36.4%,差异显著(P<0.05)。p38、p-p38表达与临床分期有关,与患者年龄、肿瘤大小无明显相关性。Westernblot也证实,p38、p-p38在有淋巴结转移患者中表达高于无淋巴结转移者(P<0.05)。结论:p38、p-p38表达与乳腺癌淋巴结转移有关,p38MAPK信号传导途径在乳腺癌侵袭与转移中起重要作用。Objective： To investigate the expression of p38 mitogen-activated protein kinase （p38 MAPK） and its relationship with lymph node metastasis and other clinicopathological features in breast invasive ductal carcinoma. Methods： The expressions of p38 MAPK and phosphorylated p38 （p-p38） were determined by immunohistochemical S-P method in 60 cases of breast invasive ductal carcinoma and by Western blot in 30 cases of breast invasive ductal carcinoma and 5 paratumor normal tissues, The relationship between the expressions of p38 MAPK and p-p38 and the clinicopathological features was analyzed by chi-square test and t test. Results： The p38 protein was mainly located in the cytoplasm of epithelial cells in breast cancer and normal breast tissue, and the p-p38 protein was mainly located in the nuclear in breast cancer. The positive expression rotes of p38 and p-p38 in breast cancer with lymph node metastasis were significantly higher than those in breast cancer without lymph node metastasis （p38 76.3% vs. 40.9%, P 〈 0.01 ; p-p38 68.4% vs. 36.4%, P 〈 0.05 ）. The expressions of p38 and p-p38 were correlated with the clinical stages, but no relationship was found between their expressions and the patient＇s age and tumor size. Conclusion： The expressions of p38 and p-p38 are correlated with lymph node metastasis in breast cancer, and p38 MAPK signaling pathway plays an important role in the invasion and metastasis of breast cancer.

关 键 词：P38蛋白 乳腺癌 磷酸化 转移 

分 类 号：R737.9[医药卫生—肿瘤]