三苯氧胺联合塞来昔布预防大鼠乳腺癌发生的实验研究  

Chemopreventive Effect of Tamoxifen Combined with Celecoxib on DMBA-induced Breast Cancer in Rats

作　　者：康华峰[1] 王西京[1] 刘小旭[1] 代志军[1] 薛锋杰[1] 薛兴欢[1] 

机构地区：[1]西安交通大学医学院第二附属医院肿瘤外科,陕西西安710004

出　　处：《癌症》2006年第11期1346-1350,共5页Chinese Journal of Cancer

基　　金：陕西省科技计划项目(No.2003K10-G40)~~

摘　　要：背景与目的:三苯氧胺能有效预防乳腺癌的发生,但并非全部有效。多种肿瘤细胞表达环氧化酶-2(cyclooxygenase-2,COX-2),其表达与肿瘤的发生发展有关。本研究观察三苯氧胺联合COX-2选择性抑制剂塞来昔布对化学致癌剂7,12-二甲基苯蒽(7,12-dimethybenzanthracene,DMBA)诱发的大鼠乳腺癌形成的影响。方法:DMBA油剂灌胃制备大鼠乳腺癌模型,每组30只大鼠,分为单纯诱癌组、三苯氧胺组、塞来昔布组和联合药物组4组,观察各组肿瘤发生率、潜伏期、肿瘤数目及体积的差异。结果:(1)乳腺肿瘤发生率三苯氧胺组(48.15%,13/27)、塞来昔布组(50.00%,14/28)低于单纯诱癌组(85.71%,24/28),高于联合药物组(21.43%,6/28);(2)乳腺肿瘤发生时间三苯氧胺组[(97.54±1.85)天]、塞来昔布组[(96.79±2.89)天]晚于单纯诱癌组[(89.50±5.99)天],早于联合药物组[(103.67±3.39)天];(3)乳腺肿瘤数目三苯氧胺组[(1.77±0.73)个]、塞来昔布组[(1.71±0.61)个]小于单纯诱癌组[(3.50±1.62)个],大于联合药物组[(1.17±0.42)个];(4)乳腺肿瘤体积三苯氧胺组[(1.78±0.71)cm3]、塞来昔布组[(2.05±1.04)cm3]小于单纯诱癌组[(6.42±3.96)cm3],大于联合药物组[(0.71±0.96)cm3],且均有统计学意义(均为P<0.05)。结论:塞来昔布和三苯氧胺能抑制DMBA诱发的大鼠乳腺癌的发生、发展,两者联合使用效果更好。BACKGROUND ＆ OBJECTIVE, Breast cancer can be prevented partly by tamoxifen. Cyclooxygenase-2 （COX-2） is expressed in many kinds of tumors, and correlated to the occurrence and progress of tumors. This study was to evaluate the chemopreventive effect of tamoxifen combined with celecoxib, a COX-2 selective inhibitor, on 7,12-dimethylbenz anthracene （DMBA）-induced breast cancer in rats. METHODS. DMBA was irrigated into the stomach of SD female rats to build breast cancer model. The rats were divided into 4 groups： control group, tamoxifen group, celecoxib group, and combination group; each group contained 30 rats. Tumor occurrence, latency period, number and volume of breast cancer were observed. RESULTS： The tumor occurrence rates in tamoxifen group （48.15%, 13/27） and celecoxib group （50.00%, 14/28） were lower than that in control group （85.71%, 24/28）, and higher than that in combination group （21.43%, 6/28）. The latency periods of tamoxifen group [（97.54± 1.85） days] and celecoxib group [（96.79±2.89） days] were longer than that of control group [（89.50±5.99） days], and shorter than that of combination group [（103.67±3.39） days]. The tumor numbers of tamoxifen group （1.77±0.73） and celecoxib group （1.71±0.61） were less than that of control group （3.50±1.62）, and more than that of combination group （1.17±0.42）. The tumor volumes of tamoxifen group [（1.78±0.71） cm^3] and celecoxib group [（2.05±1.04） cm^3] were smaller than that of control group [（6.42±3.96） cm^3], and larger than that of combination group [（0.71±0.96） cm^3]. All differences were significant （P〈0.05, respectively）. CONCLUSION, Celecoxib and tamoxifen could effectively prevent the occurrence of DMBA- induced breast cancer in rats; the combination of them has better chemopreventive effect.

关 键 词：乳腺肿瘤 化学预防 三苯氧胺 环氧化酶-2 塞来昔布 

分 类 号：R737.9[医药卫生—肿瘤]