液相色谱-串联电喷雾离子阱质谱法鉴定大鼠尿液中大豆黄素的羟基化代谢产物及其硫酸酯轭合物  

Identification of hydroxylate metabolites of daidzein and its sulfate conjugates in rat urine by LC-ESI/MS^n

作　　者：韩凤梅[1] 戈宝莹[1] 陈怀侠[1] 陈勇[1] 

机构地区：[1]湖北大学中药生物技术湖北省重点实验室,湖北武汉430062

出　　处：《药学学报》2006年第10期1004-1009,共6页Acta Pharmaceutica Sinica

基　　金：湖北省杰出青年基金资助项目(2002AC004);中药生物技术省重点实验室开放基金资助项目(2004CTM001).

摘　　要：目的鉴定大豆黄素在大鼠体内的羟基化及其结合形代谢产物。方法SD大鼠分别单剂量给药500mg.kg-1,收集0~24 h尿样。尿样经SPE ODS C18固相萃取柱纯化后,用LC-ESI/MSn对尿样中的代谢物分别进行选择离子监测(SIM)和多级质谱(MSn)分析。结果在大鼠尿中检测到几种尚未在国内外报道过的羟基化代谢产物及其硫酸酯轭合物。结论LC-ESI/MSn法可以快速、简捷、准确地鉴定大豆黄素在大鼠体内羟基化及其结合形代谢产物。Aim To identify the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine. Methods Urine samples from 0 -24 h were collected after single ig dose of 500 mg · kg^-1 daidzein to each of six rats. The urine samples were purified by SPE column （SPE C18 ） and analyzed with liquid chromatographic-tandem eleetrospray ionization ion trap mass spectrometry （LC-ESI/MS^n） for potential metabolites. Results Several new hydroxylate metabolites and its sulfate conjugates were found and identified in rat urine. Conclusion LC-ESI/MS^n is proved to be a simple, rapid, sensitive and specific technique for identification of the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.

关 键 词：大豆黄素 液相色谱-质谱联用 代谢产物 

分 类 号：R917[医药卫生—药物分析学]