抗血管生成物质凝血酶敏感蛋白在非小细胞肺癌组织中的表达  

Study on the expression of thrombinsen sitive protein in primary non-small cell lung cancer

作　　者：董济民[1] 陈锋园[1] 何积银[2] 李秀霞[2] 

机构地区：[1]西安市中心医院肿瘤内科 [2]西安交通大学医学院附属第二医院呼吸内科,710003

出　　处：《临床肿瘤学杂志》2006年第10期739-743,共5页Chinese Clinical Oncology

摘　　要：目的:探讨凝血酶敏感蛋白(TSP)的表达及微血管密度(MVD)改变与非小细胞肺癌(NSCLC)的关系。方法:用S-P免疫组织化学技术,检测了75例NSCLC癌组织和40例肺癌旁组织中TSP1和TSP2的表达及MVD的改变。结果:NSCLC癌组织中存在活跃的血管生成;MVD与NSCLC侵袭转移和临床分期有关。75例肺癌组织中TSP1和TSP2阳性表达的图像分析灰度值160·00±4·10和153·10±4·80明显高于40例肺癌旁组织阳性表达的图像分析灰度值140·20±5·20和146·70±4·50(P<0·05);TSP1和TSP2阳性表达与NSCLC患者的病情进展和转移明显有关。TSP1和TSP2表达与MVD成负相关(r=-0·940,-0·864,P<0·01),且TSP1起更重要的作用。结论:TSP1和TSP2与NSCLC的血管生成有密切关系。TSP1和TSP2及MVD是临床预测NSCLC病情进展和侵袭转移的重要标志。Objective ：To explore the relationship between the expression of thrombinsen sitive protein （TSP） and angiogenesis in non-small cell lung cancer （NSCLC）. Methods：By using SP immunohistoehemistry stainning method, expression of TSP 1 and TSP 2 and microvessel counting were evaluated in surgically resected specimens from 75 patients with NSCLC and 40 adjacent normal tissues using the anti-TSP 1 , anti-TSP 2 and anti-CD34 monoclonal antibodies respectively. Results-Active angiogenesis was observed in NSCLC tumor tissues. The microvessel density （MVD） wasrelated to metastasis and the clinical stage of NSCLC. TSP 1 , TSP 2 expressed mainly in the cytoplasm, nuclear and extracellular matrix. The expression of TSP 1 and TSP 2 in 75 tumor tissues was significantly higher than that in 40 adjacent normal tissues 160.00±4.10 versus 153.10±4.80 and 140.20±5.20 versus 146.70± 4. 50, respectively, （P〈0.05 ）. The expression of TSP 1 and TSP 2 in NSCLC patients was related to the clinical stage and metastasis of NSCLC. There was a negative correlation between TSP 1, TSP 2 expression and MVD （ r =-0.940,-0.864, respectively, P〈0.01 ）. TSP 1 plays a more important role. Conelusion：TSPland TSP 2 are closely correlated to the angiogenesis in NSCLC. TSP 1 and TSP 2, together with MVD, may serve as a clinical predictor for the metastasis of NSCLC.

关 键 词：非小细胞肺癌 凝血酶敏感蛋白 微血管密度 免疫组织化学 图像分析 

分 类 号：R734.2[医药卫生—肿瘤]