人乳腺癌细胞原代培养体外药敏试验研究  

Study of chemosensitivity for human breast cancer cells cultivated in primary generation in vitro

作　　者：李艳芬[1] 田海梅[1] 陈国际[2] 曹冬艳[1] 刘毅[1] 李茉[1] 王小兵[1] 刘朝阳[1] 梁智[1] 程冬婉[1] 张伟[1] 

机构地区：[1]中国医学科学院肿瘤医院肿瘤所生物检测中心 [2]中国医学科学院肿瘤医院肿瘤所腹部外科,100021

出　　处：《临床肿瘤学杂志》2006年第10期768-771,共4页Chinese Clinical Oncology

摘　　要：目的:探讨用ATP生物荧光肿瘤体外药敏检测技术(ATP-TCA)研究乳腺癌药物敏感性的异质性以及个体化治疗的可行性。方法:用ATP-TCA检测114例乳腺癌标本对13种单药或联合用药的敏感性。结果:个体之间的药物敏感性存在着明显的异质性。单药中有效的药物为紫杉醇、长春瑞滨、表阿霉素、丝裂霉素和阿霉素,敏感率分别为73·3%、64·1%、62·3%、49·9%和40·0%。联合用药(紫杉醇+表阿霉素,环磷酰胺+表阿霉素+氟尿嘧啶)的敏感率高于单药。结论:乳腺癌对抗癌药物的敏感程度存在着异质性。ATP生物荧光肿瘤药敏检测技术可用于为乳腺癌选择合适的化疗药物。Objective：To investigate the heterogeneity of chemosensitivity in breast cancer using an ATP-tumor chemosensitivity assay （ATP-TCA） and the feasibility of individual chemotherapy. Methods：ATP-TCA was used to determine the effect of 13 single or combined cytotoxic drugs in surgical specimens from 114 patients with breast cancer cancer. Results：There were significant differences in chemosensitivity among individuals. The effective drugs in the experiment were Paclitaxel, Vinorelbine, Epirubicin, Mitomycin and Adriamycin, the rates of chemosensitity of which were 73.3% , 64. 1% , 62. 3% , 49. 9% and 40. 0% of specimens, respectively. Combination of chemotherapy （Paclitaxel ＋ Epirubicin, Cyclophosphamide ＋ Epirubicin ＋ 5-Fluorouracil） was found to be more effective than relative drugs alone. Conclusion：The chemosensitivity to different drugs in breast cancer cells was heterogeneous. ATPTCA is an effective method for screewing appropriate drugs against breast cancer.

关 键 词：ATP生物荧光肿瘤药敏检测技术 乳腺癌 化疗药物敏感性 

分 类 号：R737.9[医药卫生—肿瘤]