hTERT、c-fos、c-jun在成釉细胞瘤中的表达及意义  

Expression and significance of hTERT, c-fos and c-jun in ameloblastoma

作　　者：钟鸣[1] 刘敬东[1] 王洁[1] 刘洁[2] 李乐[1] 侯琳[3] 张波[3] 

机构地区：[1]中国医科大学口腔医学院口腔病理科 [2]中国医科大学中心实验室,辽宁沈阳110002 [3]北京大学健康科学中心病理系,北京100083

出　　处：《上海口腔医学》2006年第5期461-465,共5页Shanghai Journal of Stomatology

基　　金：辽宁省自然科学基金(20032057)~~

摘　　要：目的:探讨c-fos、c-jun及hTERTmRNA在人成釉细胞瘤(AB)中的表达及其临床意义。方法:用原位杂交法检测AB中c-fos、c-jun和hTERTmRNA的表达,同时选取正常口腔黏膜7例、牙源性角化囊性瘤(KCOT)16例作为对照。采用SPSS10.0统计软件包进行χ2检验和Fisher确切概率法及Kendall相关分析。结果:正常口腔黏膜无c-fos、c-junmRNA的表达,hTERTmRNA仅在1/7例正常上皮中表达。c-fos、c-junmRNA和hTERTmRNA在KCOT上皮阳性表达率分别为72.7%(8/11)、35.7%(5/14)和87.5%(14/16)。AB中,c-fosmRNA阳性表达率为91.5%(43/47)、c-junmRNA阳性表达率较低,为26.7%(12/45),而hTERTmRNA高达94.0%(47/50)。c-fos和hTERTmRNA在正常口腔黏膜、KCOT及AB3组间的表达差异显著(P<0.001),而c-jun的表达在各组间未见显著差异。Kendall相关分析表明,c-fos与hTERTmRNA呈正相关(rk=0.024,P<0.05)。结论:c-fos基因在AB的发生、发展及细胞的增殖中起重要作用,且可能为构成AP-1的一个主要亚基,参与AB中端粒酶活性的调节。PURPOSE： To investigate the expression of c-fos, c-jun and hTERT mRNA in human ameloblastoma （AB） and to determine their cllnicobiological significance. METHODS： In situ hybridization was performed to examine the expression of c-fos, c-jun and hTERT mRNA in AB and 7 cases of normal oral mucosa （NOM）, 16 cases of keratocystic odontogenic tumor （KCOT） were selected as controls, SPSS10.0 software package was used to perform X^2 test, Fisher＇s exact probability and Kendall correlation analysis. RESULTS： There was no expression of c-fos and c-jun mRNA in NOM, while hTERT mRNA expressed positively only in 1/7cases of NOM. c-fos, c-jun and hTERT mRNA expressed positively in KCOT and AB with different degrees. No significant difference at c-jun mRNA levels between KCOT or AB and NOM was found, Kendall correlation analysis showed positive correlation between c-fos and hTERT mRNA （rk=0,024, P〈0.05）. CONCLUSION： c-fos gene plays an important role in the occurrence, development and proliferation of AB, possibly through acting as a cardinal subunit of AP- 1 to participate the regulation of the telomerase activity,

关 键 词：成釉细胞瘤 c—los C-JUN 端粒 反转录酶 

分 类 号：R739.8[医药卫生—肿瘤]