超声加热诱导舌鳞癌Tca8113细胞凋亡的机制探讨  

A study on the mechanism of inducing apoptosis of Tca8113 cells by means of ultrasound hyperthermia

作　　者：任国欣[1] 郭伟[2] 叶冬霞[2] 沈国锋[1] 白景峰[1] 李生娇[2] 陈亚珠[1] 

机构地区：[1]上海交通大学生命科学与技术学院生物医学仪器研究所,上海200240 [2]上海交通大学医学院附属第九人民医院.口腔医学院口腔颌面外科,上海200011

出　　处：《上海口腔医学》2006年第5期507-511,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30471898);上海市科学技术委员会科研基金(041111041);上海交通大学-上海第二医科大学合作基金(03-02)~~

摘　　要：目的:通过检测超声加热处理后Tca8113细胞凋亡的主要相关参数变化,探讨超声加热诱导肿瘤细胞凋亡的机制。方法:应用超声热辐射系统,分别对Tca8113细胞进行不同温度、时间的加热处理,流式细胞仪连续检测42℃超声加热10min后Tca8113细胞早期凋亡和继发性坏死的动态变化,以及不同处理组的线粒体跨膜电位(ΔΨm)、Caspase-3活性的变化。采用SAS6.12统计软件包进行方差分析,比较各组均数间的显著性水平。结果:42℃超声加热(10min)Tca8113细胞后1h,即检测到细胞早期凋亡,6～8h达到高峰,以后迅速下降,12h后保持在较低水平,细胞的继发性坏死伴随着凋亡出现,在高水平保持一段时间,10h后开始下降。其与早期凋亡呈正相关(r=0.7909,P=0.0064);无论温度梯度(38℃～44℃,10min)还是时间梯度(42℃,10min～60min)的超声加热,均能导致ΔΨm显著降低(P<0.01)、Caspase-3活性显著升高(P<0.01),且两者具有显著相关性(温度梯度组r=0.89189,P=0.0029,时间梯度组r=0.9679,P=0.0003)。结论:超声加热能诱导Tca8113细胞凋亡,导致ΔΨm降低、Caspase-3活性水平升高。说明超声加热通过线粒体-Caspase途径,诱导Tca8113细胞凋亡。PURPOSE： To investigate the mechanism of apoptosis in Tca8113 cells induced by ultrasound hyperthermia by detecting changes in related index. METHODS： Tca8113 cells were treated in vitro by ultrasound hyperthermia in different heating temperatures （38℃ to 44℃,10 minutes） and heating times （42℃,10 to 60 minutes）, and then the dynamic changes of early apoptosis and secondary necrosis treated by 42℃ for 10 minutes were assayed to detect A^m and Caspase-3 levels of different groups by flow cytometry （FCM）. The means of each group were compared by ANOVA with SAS6.12 software package. RESULTS： After heated by ultrasound in 42℃ for 10 minutes, the early apoptosis of Tca8113 was detected. The apoptosis index reached its highest level at the 6^th to 8^th hour, then decreased rapidly and maintained in a lower level after 12 hours.The level of secondary necrosis increased with the level of early apoptosis, but kept in a higher level until the 10^th hour, the level of secondary necrosis correlated with that of the early apoptosis（r=0.7909, P=0.0064）. The fraction of cells with low mitochondria membrane potential and increased activity of Caspase-3 were detected either in the heating-temperature grads group or in the heating-time grads group, which showed significant relationship between the two apoptosis related indes （r=0.89189,P=0.0029 in thd heating-tempcrature grads groups,r=0.9679,P=0.0003 in the heating-time grads group）.CONCLUSION：Tea8113 cells devcloped apoptosis after heated by ultmsound byperthermin.along with △ψm leyel decneasing and Caspase-3 aetivity inereasing.Ultrasound byperthermia induees apoplosis of Tca8113 cells by thd mitochomlmm-Caspase pathway.

关 键 词：TCA8113细胞 凋亡 线粒体膜电位 CASPASE-3 超声加热 

分 类 号：R739.8[医药卫生—肿瘤]