乌司他丁对大鼠心肺复苏后脑水肿和超微结构的影响  

Effects of ulinastatin on changes of cerebral edema and the ultrastructure of brain after cardiopulmonary resuscitation in rats

作　　者：李章平[1] 陈寿权[1] 于华众[2] 王姗姗[1] 方周溪[3] 王万铁[4] 王卫[4] 黄唯佳[1] 程俊彦[1] 

机构地区：[1]温州医学院第一附属医院急诊科,浙江温州325000 [2]温州医学院第二附属医院内科,浙江温州325000 [3]温州医学院电镜室,浙江温州325000 [4]温州医学院病理生理学教研室,浙江温州325000

出　　处：《温州医学院学报》2006年第5期434-438,共5页Journal of Wenzhou Medical College

基　　金：温州市科技计划重点项目(Y2004A002)

摘　　要：目的:研究乌司他丁对心肺复苏后早期脑水肿和脑超微结构的变化的影响。方法:建立大鼠心肺复苏模型,120只大鼠随机分成对照组、复苏组和药物组(每组再按时间分即刻、1/2 h、3 h、6 h、9 h五亚组),检测心肺复苏后各时相脑组织水含量及伊文氏兰(EB)含量的变化,以电镜观察各时相脑组织超微结构。结果:复苏组心肺复苏后1/2 h、3 h、6 h、9 h脑组织水含量持续增加,6 h后EB含量升高,电镜观察各时间点见细胞水肿,6 h后V-R间隙明显扩大;药物组仅于复苏后1/2、3 h有脑组织水含量的增加,6 h后水含量回降,各时间点EB含量无明显升高,复苏后6 h开始超微结构改变明显减少。结论:乌司他丁可通过抑制脑水肿,对大鼠心肺复苏后早期脑损伤起到一定的保护作用。Objective：To study the effect of ulinastatin on changes of cerebral edema and the ultrastructure of brain at different time points after early cardiopulmonary resuscitation （CPR） in rats. Methods： The animal model of cardiac arrest （CA） was established. One handred and twenty male Sprague Dawley （SD） rats were randomly divided into 15 groups（rF-8, in each） ： control group and CPR group and drug group, which were further divided into 5 subgroups respectively on the base of time. The contents of Evens blue （EB） and brain water in cerebral tissue were assayed,and the ultrastructure of brain was observed. Results： The brain edema was obvious at 1/2 h after CPR in CPR group （P〈0.05）, and was still elevated at 9 h （P〈0.05）. EB content was higher at 6 h and 9 h than that in normal control group and control group （P〈0.05）. In animals treated with ulinastatin, brain water content was increased （P〈0.05） at 1/2 h to 3 h after CPR, and was comeback after 6 h. EB content didn＇t change significantly. The observation of the ultrastructure in brain tissue showed that the injury was relieved obviously in the ulinastatin group than that in the CPR group. Conclusion： CPR results primarily in cytotoxic edema, subsequently with the increased permeability of blood-brain barrier, vasogenic edema plays a critical role in formation of brain edema. Ulinastatin can alleviate the cerebral edema and protect from cerebral damnification after early CPR in rats.

关 键 词：心肺复苏 脑水肿 超微结构 乌司他丁 

分 类 号：R541.7[医药卫生—心血管疾病]