托吡酯对戊四氮点燃慢性癫大鼠脑海马胶质细胞增生的影响  

Effect of Topiramate on brain hippocampus reactive gliosis in pentylenetetrazol induced chronic epilepsia rats

作　　者：徐惠琴[1] 朱遂强[2] 郑荣远[1] 王开颜[2] 李安乐[3] 杨学志[1] 

机构地区：[1]温州医学院第一附属医院神经内科,浙江温州325000 [2]华中科技大学同济医学院附属同济医院神经内科,湖北武汉430000 [3]温州医学院动物实验中心,浙江温州325035

出　　处：《温州医学院学报》2006年第5期451-454,共4页Journal of Wenzhou Medical College

摘　　要：目的:探讨脑海马胶质细胞增生在癫发生、发展中的作用及托吡酯对其的影响。方法:采用戊四氮制备慢性癫模型,利用托吡酯干扰,选取不同时间点,观察大鼠行为学变化及胶质纤维酸性蛋白在海马回中的表达(免疫组织化学染色)。结果:托吡酯组点燃率在27 d明显低于模型组;模型组及托吡酯组胶质纤维酸性蛋白表达增加,并随时间延长更加明显;而不同时间点该表达的增加程度,托吡酯组均低于模型组。结论:胶质纤维酸性蛋白表达的增加,说明出现了胶质细胞活化及脑可塑性变化,而托吡酯明显地抑制了该表达的增加。Objective：To Study the effect of brain hippocampus reactive gliosis in the process of occurrence and development of epilepsy and the effect of Topiramate （TPM） on it. Methods： A model of chronic epilepsy was established with pentylenetetrazol. Rats were randomly divided into 3 groups：control group（C group）, PTZ group （P group, P1 - 10 d, P2-20 d and P3 - 27 d）and TPM＋PTZ group（TP group, TP1 -10 d, TP2-20 d and TP3 -27 d）, The behavior and expression of Glial fibrillary acidic protein （GFAP） by immunohistochemical staining were observed 10, 20, 27 days after using PTZ. Results： There was significant difference in behavior between group P and TP after 4 weeks. Expression of GFAP increased in group P and TP （P〈0.05）; and continuously increased along with time.and the expression in P group is more higher than in group TP at different time-points （P〈0.05）. Conclusion： The increasing of Expression of GFAP in hippocampus indicates that reactive gliosis occurs and neural plasticity maybe an important mechanism of epileptogenesis in the process of PTZ kindling rats.Expression of GFAP is inhibited markedly by TPM, which indicates that TPM can influence expression of GFAP and may influence nerve plasticity.

关 键 词：癫痫 脑可塑性 托吡酯 戊四氮 胶质纤维酸性蛋白 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R332[医药卫生—临床医学]