新生Long Evans大鼠视网膜小胶质细胞原代培养及鉴定  

Culture and identification of neonate rat retinal microglia cells in vitro

作　　者：吴楠[1] 王艳华[1] 刘东宁[1] 王一[1] 

机构地区：[1]第三军医大学西南医院全军眼科中心,中国重庆市400038

出　　处：《国际眼科杂志》2006年第5期1039-1041,共3页International Eye Science

摘　　要：目的:建立稳定高产的新生Long Evans大鼠视网膜小胶质细胞(retinamicrogliacellRMG)体外培养、分离的方法。方法:取出生3d内的LongEvans新生大鼠视网膜神经上皮层进行混合细胞培养,10～12d后振荡分离,纯化培养得到小胶质细胞;小胶质细胞的特异性标记IB-4及CD11b进行组化及细胞免疫荧光鉴定,扫描电镜观察细胞表面突起。结果:得到纯度较高的小胶质细胞,IB-4组化及CD11b细胞免疫荧光鉴定纯度分别为94.5%和95.8%,扫描电镜观察细胞表面为棘状突起,明显与巨噬细胞的细胞表面区别。结论:本方法可以得到纯度高的视网膜小胶质细胞。AIM： To establish a culture method of Long Evans neonate rat retinal microglia cells（RMGs） in vitro. METHODS： Mixed glial cells of Long Evans rats （postnatal 〈3d） were cultured for 10-12 days, then RMGs were purified by shaking in 37℃ swing bed and identified by specific microglia marker 113-4 and CD11b, and cell surface of RMC-6 were observed by Scanning EM. RESULTS： Purity of RMGs were 94.5% and 95.8% by cell immunochemistry identification of I B-4 and CD11b, cell surface showed spinous processes of RMGs, contrasting the smooth surfaces of macrophages. CONCLUSION： Based on these data, a method is presented that proposes to use the microglial cell to effectively deliver therapeutic compounds to retinal disease.

关 键 词：视网膜小胶质细胞 细胞培养 LONG EVANS大鼠 

分 类 号：R77[医药卫生—眼科]