莪术醇修饰的肿瘤疫苗抗MFC效应的研究  

Anti-MFC effect of curcumol -modified tumor cells vaccine

作　　者：徐立春[1] 边可君[1] 卜平[1] 许祥裕 周娟[1] 刘志敏[1] 孙振华[1] 陈平[3] 

机构地区：[1]扬州大学中西医结合研究所,江苏扬州225001 [2]南京军事医学研究所,江苏南京210029 [3]江苏省苏北人民医院,江苏扬州225001

出　　处：《现代肿瘤医学》2006年第11期1357-1359,共3页Journal of Modern Oncology

基　　金：江苏省卫生厅重大科研课题资助项目(编号K200511)

摘　　要：目的:研究中药莪术提取的有效成分-莪术醇生物修饰构建的肿瘤细胞疫苗对荷瘤(MFC)小鼠的免疫功能变化及治疗效应。方法:用现代生物学技术修饰构建瘤苗,观察莪术醇-MFC细胞瘤苗体外激活脾淋巴细胞的能力,体内成瘤性变化及作为瘤苗对荷瘤胃癌的小鼠治疗作用。结果:莪术醇-MFC细胞瘤苗在体外能有效诱导免疫细胞活化增殖和特异性CTL活性的产生,体内的成瘤能力明显下降,免疫接种瘤苗后对荷瘤鼠胃癌小鼠有较好的治疗效果。结论:莪术醇修饰的肿瘤细胞瘤苗能明显增强其免疫原性、对荷瘤胃癌小鼠有较好的治疗效应。Objective：To explore the anti - MFC effect on gastric cancer （MFC） by tumor cell vaccine modified with Curcumol. Methods： After the MFC cell line was established by modified of curcumol , the capacity of curcumol - MFC cells to activate spleen lymphocytes was determined in vitro and the therapeutic effects of the cells being used as vaccine on gastric cancer were investigated in mice . Results： Curcumol modified MFC vaccine effectively induced the activation and proliferation of the spleen cell and the production of CTL cytotoxictity in vitro . The tumorogenicity of curcumol - MFC cells was remarkably low in vivo . The curcumol - MFC cells could exert good effection gastric cancer in the mice. Conclusion： The immuogenicity is increased by curcumol modified MFC tumor cells and can effectively inhibit the growth of MFC in the host .

关 键 词：莪术醇 MFC 生物构建疫苗 治疗胃癌效应 

分 类 号：R73-362[医药卫生—肿瘤]