石蜡包埋的石棉肺癌组织的基因组DNA的快速提取法  

Rapid extraction of genomic DNA from paraffin embedded asbestos related lung cancer tissues

作　　者：付德辰[1] 刘秉慈[1] 缪庆[1] 王海华[1] 尤宝荣[1] 马旭 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所

出　　处：《卫生研究》1996年第4期204-205,共2页Journal of Hygiene Research

基　　金：本课题为中国预防医学科学院科研基金资助项目

摘　　要：建立了从少量石蜡包埋的组织切片中快速提取石棉肺癌组织中的基因组ＤＮＡ的水煮脱蜡法，克服了传统的二甲苯脱蜡法的周期长、成本高和毒性大的缺点。所得到的基因组ＤＮＡ中，长片段占较大的比例，总量可足够用作１０～２０次ＰＣＲ扩增反应的模板。rapid method of extraction of genomic DNA from small amount of paraffin embedded tissues was developed in this experiment. This method overcame the deficiency of the classical method, which is time consuming and with severe toxicity. The genomic DNA prepared in this method had many long fragments and they could be used to prepare temples for PCR or be used in other molecular biology research.

关 键 词：石蜡包埋 石棉 DNA提取 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]