硒和VE对大鼠串珠镰刀菌素毒性的影响  

Effect of selenium and vitamin E on the toxicity of moniliformin in rats

作　　者：顾履珍[1] 田园[1] 哈鹏程[1] 许洁[1] 李卫东[1] 周燕[1] 夏奕明 

机构地区：[1]中国预防医学科学院营养与食品卫生研究所

出　　处：《卫生研究》1996年第4期228-231,共4页Journal of Hygiene Research

基　　金：卫生部科研基金资助项目和"八五"攻关科研基金资助项目

摘　　要：体重７０～８０ｇＷｉｓｔａｒ雄性大鼠５４只随机分成３组，分别饲以低硒低ＶＥ、适硒低ＶＥ和适硒适ＶＥ半合成饲料。４周后每组中的一半动物用串珠镰刀菌素（ＭＦ）２０ｍｇ／（ｋｇ·ｄ）灌胃，另一半用去离子水灌胃作对照。实验持续７周。结果显示：给ＭＦ的动物心肌组织中的硒浓度、琥珀酸脱氢酶、丙酮酸脱氢酶、超氧化物歧化酶、细胞色素Ｃ氧化酶和谷胱甘肽过氧化物酶活力均与对照组无显著差异，但在睾丸组织中硒浓度和谷胱甘肽磷脂氢过氧化物酶活力只分别相当于对照组的５０％和１０％。光镜和电镜检查均未发现ＭＦ会引起心肌病变，但睾丸严重萎缩，造精细胞明显减少。此结果说明ＭＦ的致毒靶器官为睾丸。ifty four male Wistar rats of 70～80g body weight were randomly divided into 3 groups and were fed with semisynthetic diets of low Se low VE, adequate Se low VE and adequate Se adequate VE respectively. After 4 weeks, half of the animals in each group were given 20 mg moniliformin (MF)/(kg·d) by gavage and the other half deionized water as controls. The total experimental period was 11 weeks. The results show that the Se concentration, the activities of SDH(succinate dehydrogenase), PDH(pyruvate dehydrogenase), SOD(superoxide dismutase), CCO(cytochrome oxidase) and GPX(glutathione peroxidase) in the heart muscle of animals given MF were not different from those of the control animals; whereas the Se concentration and PHGPX activity in the testes of animals given MF were only 50% and 10% respectively of those in the control animals. No pathological changes in heart muscle was observed either by light or electronic microscope in animals given MF. However their testes showed serious atrophy and the number of spermatogenic cells reduced significantly. These results indicate that testicle is the target organ of MF. Se and VE have no protective effect against the toxicity of moniliformin.

关 键 词：串珠镰刀菌素 硒 维生素E 克山病 

分 类 号：R542.305[医药卫生—心血管疾病]