Bcl-2,Survivin,CD_(44)V_6在宫颈癌组织中的表达及其与HPV_(16/18)感染的相关性分析  

Relationship between apoptosis with Bcl-2 and Survivin,CD_(44)V_6and HPV_(16/18) infection in uterine cervical carcinogenesis

作　　者：赵富玺[1] 穆雅琴[1] 郭俊晨[1] 刘润花[1] 

机构地区：[1]山西大同大学医学院

出　　处：《大同医学专科学校学报》2006年第4期1-5,15,共6页Journal of Datong Medical College

基　　金：山西省自然科学基金资助(20051115)

摘　　要：目的:研究Bcl-1、Survivin、CD44V6在宫颈癌组织中的表达及其与细胞凋亡、HPV16/18感染的相关性,以探讨宫颈癌的发生机制,指导其诊断预后;方法:采用免疫组织化学方法对宫颈癌、宫颈上皮内瘤样病变(CIN)和正常宫颈组织的石蜡标本进行Bcl-2、Survivin、CD44V6的检测,同时采用TUNEL法和PCR技术检测细胞凋亡指数和HPV16/18感染情况;结果:Bcl-2、Sur-vivin、CD44V6在宫颈癌组织中的阳性率与CIN、正常宫颈组织有显著性差异,且随着临床分期病理分级的升高,阳性率差异有显著性,其表达率与淋巴结转移有关。HPV16/18在正常宫颈、CIN和宫颈癌组织中的阳性率逐渐升高,差异有显著性,但与临床分期病理分级淋巴转移无关。AI在宫颈癌中显著高于CIN和正常宫颈组织,差异有显著性,且其与宫颈癌的临床分期病理分级淋巴结转移有关。Bcl-2、Survivin和AI均与HPV16/18感染有相关性;结论:Bcl-2、Survivin、CD44V6在宫颈癌组织中的异常表达与HPV16/18感染有关,与宫颈癌的恶变程度有关,可作为筛查宫颈癌和判断预后的有利指标。Objective：To investigate the relationship between apoptosis with Bcl-2 and Survivin,CD44V6 and HPV16/18 infection in uterine cervical carcinogenesis. Method： Formalin-fixed, paraffin-embedded samples from cervical carcinomas, cervical intraepithelial neoplasia and normal cervical tissues were studied. Using streptavidin-biotin roxidase （s-p） immtmohistochemical technique, the authors examined the expression of Bcl-2, Survivin and CD44V6 in these samples. The number of apoptosis cells was assessed in situ by TdT-mediated dUTP-biotin end labeling （TUNEL） method. The infection of HPV type 16,18 DNA were determined by PCR. Result： There were significant differences for Bcl-2, Survivin, CD44 V6 and AI between carcinomas, CIN and normal cervices, respectively. Expression of Bcl-2, Survivin, CD44V6 and AI were corrleated with tumor grades, clinical stages and lymph node metastasis. The positive rate of HPV16/18 was significant difference between cervical carcinomas, CIN and nornal tissues and was not associated with tumor grades, clinical stages and lymph node metastasis. The infection of HPV16/18 was associated with the expression of Bcl-2, Survivin and AI, respectively. Conclusion：The innormal expression of Bcl-2, Survivin and CD44V6 are associated with the infection of HPV16/18. They possibilly become useful indexs for the primary screening and prognosis of cervical carcinomas.

关 键 词：BCL-2 SURVIVIN CD44V6 细胞凋亡 HPV 宫颈癌 

分 类 号：R735.7[医药卫生—肿瘤]