抗UV-B辐射菌紫外吸收代谢产物分析及其抗紫外辐射活性研究  

Analysis of the UV absorbing constituents of the metabolites from UV-B tolerance bacteria and study on its anti-ultraviolet mechanism

作　　者：王洪媛[1] 江晓路[1] 任虹[1] 梁晓婷[1] 管华诗[1] 

机构地区：[1]中国海洋大学海洋医药学院,山东青岛266003

出　　处：《中国海洋药物》2006年第4期1-5,共5页Chinese Journal of Marine Drugs

基　　金：908专项:海洋药用生物资源调查与研究(908-01-ST12)

摘　　要：目的从代谢产物的角度研究一株具有抗UV-B辐射菌KFS-9的抗紫外辐射机制。方法利用各种色谱层析技术分离纯化该菌株的紫外吸收代谢产物,并利用GC-MS技术,LC-TOF-MS技术,NIST数据库分析以及NMR,EI-MS色谱数据鉴定其有效组成成分。考察紫外吸收组分的紫外辐射保护活性。结果石油醚层萃取物含有3种不饱和脂肪酸类化合物:9-棕榈烯酸、十八碳-9,12-二烯酸、十八碳-11-烯酸,以及少量的紫外吸收化合物1,2-邻苯二甲酸。乙酸乙酯层萃取物中的高效紫外吸收组分(Ⅱ)主要为2,3-丁二醇,其中含有一组高度不饱和的芳香族类化合物。组分M具有很宽的紫外吸收峰,并对菌体具有一定的紫外辐射保护作用。结论菌株KFS-9通过产生具有紫外吸收作用的代谢产物,形成一道天然屏障,提高其对紫外照射的抗性。对大肠杆菌的紫外辐射保护实验表明,高效紫外吸收组分(Ⅱ)并不是唯一的保护剂,必然存在其它的物质及保护机制发挥作用。Objective The anti-UV-B radiation mechanism of UV-B tolerance strain KFS-9 was studied from the profile of metabolites. Methods The compounds were separated by column chromatography and their structures were elucidated based on GC-MS, LC-TOF-MS, EI-MS and NMR analyses. Results Three unsaturated fatty acids （identified as 9-hexadecenoic acid, 9,12-octadecadienoic acid and 11-octadecenoic acid） and 1,2-benzenedicarboxylic acid able to absorb ultraviolet were isolated from the petroleum ether extract of the fermentation liquid of Pantoea agglornerans KFS-9. Fraction （ Ⅱ ） was isolated from the ethyl acetate extract and was composed of 2,3-butanediol and a series of high unsaturated aroma compounds. Fraction （ Ⅱ ） had a wide absorption peak, and it could protect E. coli from UV-B damage in some sense. Conclusion Strain KFS-9 produced metabolites that were able to absorb UV to build a natural barrier and so improved the tolerance to UV radiation. The UV-B radiation protec-tion test to the E. coli also showed fraction （ Ⅱ ） was not the only protector, and there defi nitely existed other materials and mechanism to protect the strain.

关 键 词：成团泛菌 抗紫外辐射 2 3-丁二醇 UV—B 代谢产物 

分 类 号：R931.711[医药卫生—生药学] R979.6[医药卫生—药学]