β-榄香烯介入治疗兔VX2肾移植癌的实验研究  

Research of β-elemene interventional treatment on VX2 carcinoma transplanted on kidney in rabbits

作　　者：佘军军[1,2] 王子明[1] 车向明[3] 潘承恩[3] 

机构地区：[1]西安交通大学医学院第二附属医院泌尿外科 [2]西安交通大学医学院第一附属医院普通外科,陕西西安710061 [3]西安交通大学医学院第一附属医院普通外科

出　　处：《中西医结合学报》2006年第6期611-614,共4页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.30371831)

摘　　要：目的探讨β-榄香烯介入治疗对兔VX2肾移植癌的作用及其机制。方法建立兔VX2肾移植癌模型,将动物随机分为生理盐水对照组,碘油栓塞,丝裂霉素、5-氟尿嘧啶、β-榄香烯、顺铂、卡铂、阿霉素、噻替哌、环磷酰胺和长春新碱等药物介入治疗组。各组予以相应干预措施,采用螺旋CT及B超动态监测各组肿瘤体积变化并计算平均抑瘤率。治疗后1、7、14d作血液生化检查。治疗后14d采用光镜及透射电镜观察肿瘤组织的形态变化,免疫组化法检测Bax和Bcl-2的表达。结果不同药物介入治疗对兔VX2肾移植癌的效果差异有统计学意义。兔VX2肾移植癌对碘油栓塞、丝裂霉素、顺铂、卡铂高度敏感,肾功能损伤严重;对β-榄香烯、阿霉素、5-氟尿嘧啶中度敏感,β-榄香烯对肾功能损伤较轻微;对噻替哌、环磷酰胺、长春新碱耐药。光镜及透射电镜下β-榄香烯治疗组以肿瘤细胞凋亡为主,偶见细胞自溶;Bax表达显著上调(P<0.05),Bcl-2表达差异无统计学意义(P>0.05)。结论β-榄香烯介入治疗肾癌效果显著,肾脏损伤轻微,其作用机制以促进肿瘤细胞凋亡为主,Bax参与该途径。Objective： To investigate the curative effect and mechanism of β-elemene interventional treatment on VX2 carcinoma transplanted on kidney in rabbits. Methods. The rabbits were all transplanted with VX2 carcinoma on kidney. Fifty-five rabbits were randomly divided into 11 groups. Rabbits in these groups were administered interventional treatment of normal saline, iodinated oil, mitomycin, 5-fluorouracil, β-elemene, cisplatin, carboplatin, adriamycin, thiotepa, cyclophopsphamide, and vincristine, respectively. After corresponding intervention, the tumor volume in each group was measured by ultrasonography and spiral computed tomography, and the tumor growth rate （TGR） was calculated. Nenal and hepatic functions of the rabbits in each group were compared 1 day, 7 and 14 days after the interventional treatment. Morphologic change of the tumor was observed by a light microscopy and a transmission electron microscopy 14 days after interventional treatment. The expressions of Bax and Bcl-2 were measured by immunohistochemical straining. Results, There was statistical significance in the effects of different medicines intervened on VX2 kidney transplanted carcinoma. The VX2 carcinoma of rabbits had high-sensitivity to iodized oil embolism, mitomycin, cisplatin and carboplatin, which showed serious damage to the kidney function, mediumsensitivity to β-elemene, adriamycin and 5-fluorouracil, in which β-elemene showed slight damage to the kidney function, and resistance to thiotepa, cyclohosphamide and vincristine. Most tumor cells displayed apoptosis in the β-elemene interventional treatment group under light microscopy and transmission electron microscopy, and only few tumor cells displayed necrosis. The Bax expression was up-regulated （P〈0.05） and the Bcl-2 expression had no significant difference （P〉0.05） in the β-elemene interventional treatment group. Conclusion： Intervention treatment of β-elemene has significant effect on VX2 kidney transplanted carcinoma and little side effect on the kidn

关 键 词：Β-榄香烯 兔VX2肾移植癌 介入治疗 凋亡 

分 类 号：R737.11[医药卫生—肿瘤]