细胞外信号传导激酶在耐药性颞叶癫患者中的表达  

Overexpression of extracelluar signal regulated protein kinase in the temporal lobe of human drug-resistant epilepsy

作　　者：席志芹[1] 王学峰[1] 吴源[2] 孙纪军[1] 朱曦[1] 肖飞[1] 李劲梅[1] 关立峰[1] 龚云[1] 刘凤英[1] 晏勇[1] 郭珍立 栾国明[4] 张建国[4] 王玉平[5] 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室,400016 [2]广西医科大学附属医院神经内科 [3]武汉新华脑科中心神经内科 [4]首都医科大学北京天坛医院 [5]首都医科大学北京宣武医院神经内科

出　　处：《中华神经科杂志》2006年第10期691-696,共6页Chinese Journal of Neurology

摘　　要：目的通过观察细胞外信号传导激酶(extracelluar signal regulated protein kinase,ERK)及其磷酸化产物p-ERK1/2在耐药性癫患者脑组织中的表达,探讨其在癫发生、发展中的作用。方法免疫组织化学及Western blot法检测32例耐药性颞叶癫(drug-resitstance of temporal lobeepilepsy,DR-TLE)患者(颞叶24例,海马8例)手术标本中ERK1、ERK2、p-ERK1/2(thr202/thr204)的表达,并与12例对照组(颞叶9例,海马3例)进行比较。结果ERK1、ERK2、p-ERK在颞叶中的神经元和胶质细胞中均有表达,且DR-TLE中表达(0.2266±0.0613、0.2097±0.0183和0.1924±0.0054)高于对照组(0.1840±0.0023、0.1974±0.0056和0.1825±0.0063,P<0.05),Western blot分析发现同样的结果。结论ERK及其磷酸化产物在DR-TLE中表达上调,提示ERK信号传导途径可能在DR-TLE发生、发展中起一定作用。Objective To investigate expression of extracelluar signal regulated protein kinase （ERK） and phosphorylation ERK （p-ERK） in the temporal lobe from patients with DR-TLE so as to explore the possible roles of ERK in the pathogenesis of DR-TLE. Methods Expression of ERK was detected with Western blot and immunohistochemistry in 32 patients with DR-TLE （24 temporal lobe, 8 hippocampi ） , as compared with 12 controls （9 temporal lobe, 3 hippocampi） . Results ERK and p-ERK expression in DR-TLE was significantly higher （0. 2266 ± 0. 0613, 0. 2097 ± 0. 0183 and 0. 1924 ± 0. 0054, respectively） than those of controls （0. 1840 ± 0. 0023, 0. 1974 ± 0. 0056 and 0. 1825 ± 0. 0063, respectively, all P 〈0. 05）. ERK and p-ERK were mainly expressed in the cytoplasm of neuron and glial. The results of Western blot were well concordant with immunohistochemistry data. Conclusions The expression of ERK in epilepsy patients was distinct from those of control brain samples. A pathogenetic role of ERK in DR-TLE might be included.

关 键 词：癫痫 颞叶 有丝分裂素激活蛋白激酶类 磷酰化 

分 类 号：R742.1[医药卫生—神经病学与精神病学]