毛细管电泳电致化学发光法测定大豆中脯氨酸  

Aanalysis of Proline Content in Soybean by Capillory Electrophoresis

作　　者：杨丹[1] 郝再彬[1] 孙宇哲[1] 苍晶[1] 邱丽娟[2] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]中国农科院作物品种资源研究所,北京100081

出　　处：《中国粮油学报》2006年第5期148-150,共3页Journal of the Chinese Cereals and Oils Association

基　　金：国家863基金资助项目(No:2003AA207060);黑龙江省人事厅(2004年度)支持项目

摘　　要：本文介绍了一种用毛细管电泳电致化学发光测定脯氨酸含量的新方法，探讨了进样量、温度、毛细管电泳高压、缓冲液pH等条件对信号强度、迁移时间的影响。根据实验结果确定的优化电泳条件为：10mmol／L磷酸缓冲液（pH=8．0），柱温30℃，毛细管电泳高压18kV。优化条件下，脯氨酸迁移时间和峰高定量精度分别在1．68％和3．84％以内，在1～100μg／mL浓度的范围内存在良好的线性关系（r^2=0．9992），最低检出限为1μg/mL（s／n=3），该方法已成功用于大豆中脯氨酸含量的测定。This article introduces a new method for measuring the proline content of soybean by capillary electrophoresis and electro - chemiluminescdence, and explores the influence of injection quantity, temperature, high voltage of capillary electrophoresis, and pH of the buffer on the message intensity and the migration time. The optimum electrophoresis condition is confirmed based on the experimental results： 10 mmol/L phosphatic buffer （pH = 8.0）, column temperature 30%, and voltage vault 18 kV. The migration time of proline and its definition number of peak value are between 1.68% and 3.84%. There is a good linear relation between them over the range of 1 -100 μg/mL, The minimal detective content is 1 μg/mL （ s/n = 3）. The examination of proline in soybean by using this method succeed .

关 键 词：毛细管电泳电致化学发光脯氨酸 

分 类 号：O657.8[理学—分析化学]