芹菜胚性愈伤的诱导及高频植株再生体系的建立  

Embryonic Callus Induction and Plant High Efficient Regeneration System from Celery(Apium graveolens L.)

作　　者：韩清霞[1] 沈火林[1] 朱鑫[1] 陈蓉[1] 杨辉[1] 杨学妍[1] 

机构地区：[1]中国农业大学农学与生物技术学院

出　　处：《中国蔬菜》2006年第11期6-9,共4页China Vegetables

基　　金：国家自然科学基金项目(30471179);国家"948"项目(2003-Z28B-11)

摘　　要：通过对芹菜离体培养过程中激素配比、基因型、不定芽产生等条件的研究,建立了芹菜高频植株再生体系。研究表明,芹菜苗期的下胚轴最适于诱导愈伤组织,其愈伤组织诱导和继代培养的最佳培养基是MS+2,4-D1.0mg·L-1+KT1.0mg·L-1和MS+2,4-D0.5mg·L-1+BA0.2mg·L-1+CH500mg·L-1+4%甘露醇;基因型对愈伤组织和胚状体的诱导影响很大;60%以上的愈伤组织在继代培养基上可以从非胚性状态向半胚性和胚性状态转化。愈伤组织在1/2MS+1.5%蔗糖+CH500mg·L-1+KT0.25mg·L-1上获得不定芽或小试管苗;在无激素的MS或1/2MS固体培养基上继续培养不定芽或小试管苗,即可生根或长大,获得芹菜的完整再生植株。An efficient plant regeneration system was established through optimization of hormone combination, genotype, producing of adventitious bud in celery culture. The best media for callus induction and subculture were MS medium supplemented with 2,4-D 1.0 mg·L^-1 ＋KT 1.0mg·L^-1,2,4-D 0.5 mg·L^-1＋BA 0.2 mg·L^-1 ＋ CH 500 mg·L^-1 ＋4 % mannitol. Genotype was an important factor for callus and somatic embryos induction. 60 % Callus could be transfered from non-embryonic to semi-embryonic and embryonic callus. On 1/2 MS ＋ 1.5 % sucrose ＋ CH 500 mg·L^-1 ＋ KT 0.25 mg·L^-1 medium, regenerated shoots or small test tube seedlings were differentiated from Callus, embryonic callus was the easiest to differentiation and somatic embryos induction. MS or 1/2 MS medium without growth regulators was optimum for root formation of regenerated shoots.

关 键 词：芹菜 胚性愈伤 植株再生 高频 

分 类 号：S636.3[农业科学—蔬菜学]