电喷雾质谱法研究人参皂苷Rb_1和Rd与细胞色素c的非共价复合物  

Studies on Ginsenoside Rb_1 and Rd Binding to Cytochrome c by Electrospray Ionization Mass Spectrometry

作　　者：张华蓉[1] 张寒琦[1] 王玉堂[1] 李丹[1] 丁兰[1] 

机构地区：[1]吉林大学化学学院,长春130012

出　　处：《高等学校化学学报》2006年第11期2061-2065,共5页Chemical Journal of Chinese Universities

基　　金：吉林省科技发展计划项目(批准号:20050560)资助

摘　　要：用质谱法研究了人参皂苷Rb1和Rd与细胞色素c的非共价相互作用,证明主-客体之间形成了多种化学计量比的复合物,用直接计算的方法得出人参皂苷Rb1和Rd与细胞色素c形成的非共价复合物的各级解离常数KD,用竞争体系验证了计算结果,二者结果相互吻合.Electrospray ionization mass spectrometry was applied to investigating the noncovalent interaction between ginsenosides and cytochrome c. Several noncovalent complexes with different stoichiometric relationships were observed from the mass spectra. The competition experiment was performed to validate the results. The dissociation constants of the complexes obtained by different systems are consistent with each other.

关 键 词：电喷雾离子化质谱 人参皂苷 细胞色素C 非共价复合物 

分 类 号：O657[理学—分析化学]