Inhibitory effect of electro-acupuncture on apoptosis after spinal cord injury  

作　　者：Zhiying Zhang Ruishan Dang Chuansen Zhang Liang Li Congli Ren Shuang Zhou Eryu Chen 

机构地区：[1]Department of Anatomy, Second Military Medical University of Chinese PLA, Shanghai 200433, China [2]Shanghai Research Center of Acupuncture ＆ Mendian, Shanghai 201203, China [3]Department of Acupuncture and Moxibustion, Second Military Medical University of Chinese PLA, Shanghai 200433. China

出　　处：《Neural Regeneration Research》2006年第3期239-243,共5页中国神经再生研究（英文版）

基　　金：a grant of theScience and Technology Com-mission of Shanghai Municipali-ty, No. 03DZ19554-7; the Na-tional Basic Research Program,No. 2005CB523306

摘　　要：BACKGROUND： Acupuncture and electro-acupundura （EA） as complementary and alternative medicine have been applied for neurological recovery after spinal cord injury （SCI）, but the mechanism of treatment is unclear yet. OBJECTIVE： To examine and compare the effects of EA with methylprednisolone （MP） on apoptosis and expression of caspase-3 mRNA and protein in early SCI, in an attempt to provide experimental clues for clinical use of EA in the treatment of early SCI. DESIGN ： A randomized control tria SETTING ： Department of Anatomy, Second Military Medical University of Chinese PLA MATERIALS： Forty-eight adult male Sprague-Dawley （SD） rats aged 12 weeks and weighing （250±20） g were used. The experimental procedures were performed in accordance with the animal care guidelines of the National Institute of Health （NIH）. Equipments and medicine： Methylprednisolone sodium succinate （Pharmacia ＆ Upjohn N.V./S.A., Puurs, Belgium）; sodium pentobarbital （Sigma Chemical Co., St. Louis, MO）; G-6805-2 Multi-Purpose Health Device （Shanghai Medical Instruments High-TECH Co., Shanghai, China）; POD kit （Roche Molecular Biochemicals, Mannheim, Germany）; ISH Detection Kit （Wuhan Boster Biological Technology Ltd., Wuhan, China）; ABC kit （Vector Laboratories, Burlingame, CA）; light microscope （x200, Olympus, Tokyo, Japan）; HPIAS-1000 color High-definition pathological image analysis system （Qianping Imaging Engineering Company of Wuhan Tongji Medical University）. METHODS： The experiment was carried out in Department of Anatomy, Second Military Medical University of Chinese PLA between October 2004 and March 2006. ① The SCI model was established by modified Allen＇s method on T10. The force applied in traumatization was 50 g.cm. ②Forty-eight SD rats were equally randomized into four groups： sham operation （SO） group, in which animals received laminectomy only; model control （MC） group as a negative control, in which animals did not receive any treatmBACKGROUND： Acupuncture and electro-acupundura （EA） as complementary and alternative medicine have been applied for neurological recovery after spinal cord injury （SCI）, but the mechanism of treatment is unclear yet. OBJECTIVE： To examine and compare the effects of EA with methylprednisolone （MP） on apoptosis and expression of caspase-3 mRNA and protein in early SCI, in an attempt to provide experimental clues for clinical use of EA in the treatment of early SCI. DESIGN ： A randomized control tria SETTING ： Department of Anatomy, Second Military Medical University of Chinese PLA MATERIALS： Forty-eight adult male Sprague-Dawley （SD） rats aged 12 weeks and weighing （250±20） g were used. The experimental procedures were performed in accordance with the animal care guidelines of the National Institute of Health （NIH）. Equipments and medicine： Methylprednisolone sodium succinate （Pharmacia ＆ Upjohn N.V./S.A., Puurs, Belgium）; sodium pentobarbital （Sigma Chemical Co., St. Louis, MO）; G-6805-2 Multi-Purpose Health Device （Shanghai Medical Instruments High-TECH Co., Shanghai, China）; POD kit （Roche Molecular Biochemicals, Mannheim, Germany）; ISH Detection Kit （Wuhan Boster Biological Technology Ltd., Wuhan, China）; ABC kit （Vector Laboratories, Burlingame, CA）; light microscope （x200, Olympus, Tokyo, Japan）; HPIAS-1000 color High-definition pathological image analysis system （Qianping Imaging Engineering Company of Wuhan Tongji Medical University）. METHODS： The experiment was carried out in Department of Anatomy, Second Military Medical University of Chinese PLA between October 2004 and March 2006. ① The SCI model was established by modified Allen＇s method on T10. The force applied in traumatization was 50 g.cm. ②Forty-eight SD rats were equally randomized into four groups： sham operation （SO） group, in which animals received laminectomy only; model control （MC） group as a negative control, in which animals did not receive any treatm

关 键 词：TUNEL SCI 

分 类 号：R[医药卫生]