右-苯丙胺对脑缺血大鼠神经丝蛋白和微管相关蛋白表达的影响  

Effects of D-amphetamine on expression of NF and MAP2 after cerebral ischemia in rats

作　　者：李红梅[1] 肖农[1] 张晓萍[1] 

机构地区：[1]重庆医科大学儿童医院神经内科,400014

出　　处：《中国康复医学杂志》2006年第10期883-886,共4页Chinese Journal of Rehabilitation Medicine

基　　金：重庆市科委自然科学基金面上项目[渝科技字(2002)-18文]

摘　　要：目的:研究右-苯丙胺对大鼠大脑中动脉闭塞MCAO后神经丝蛋白(NF)、微管相关蛋白(MAP2)表达的影响,并在分子水平上探讨其加速神经康复的机制。方法:应用Koizumi线栓法建立单侧MCAO模型,用免疫组织化学方法及RT-PCR检测缺血周围区MAP2、NF表达的变化。结果:在MCAO1周末MAP2、NF的表达明显低于假手术组,以后逐渐增高,在第6周末其表达高于假手术组,模型+右-苯丙胺组在各时间点其表达均明显高于模型组。结论:右-苯丙胺可促进脑缺血后动物的MAP2、NF的表达,提高神经可塑性,可能为其加速神经康复的分子机制之一。Objective：To study the effects of D-amphetamine on the expression of microtubule-associated protein2 （MAP2） and neurofilament protein （NF） after middle cerebral artery occlusion （MCAO）,and explore the molecular mechanism of D-amphetamine in accelerating rehabilitation in rats with acute cerebral infarction.Method：The unilateral MCAO models were induced by using Koizumi＇s method. The changes in the expression of MAP2,NF around ischemia area was observed by reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemical techniques.Result：Compared with the sham-operated group,the expression of MAP2 and NF evidently decreased at 1 week,then increased gradually. Both the expression of MAP2 and NF proteins demonstrated statistically significant increased in D-amphetamine treatment group compared with natural recovery group all time points. Same results appeared in RT-PCR product.Conclusion：D-amphetamine effectively promotes the expression of MAP2 and NF around ischemia area, which indicates that neural plasticity is molecular mechanism of D-amphetamine in accelerating rehabilitation in rats with acute cerebral infarction.

关 键 词：脑缺血 微管相关蛋白 神经丝蛋白 右-苯丙胺 

分 类 号：R743[医药卫生—神经病学与精神病学]