阿司匹林和塞莱昔布对幽门螺杆菌的体外影响  

Effect of aspirin and celecoxib on Helicobacter pylori in vitro

作　　者：马慧霞[1] 王蔚虹[1] 胡伏莲[1] 李江[1] 

机构地区：[1]北京大学第一医院消化内科

出　　处：《世界华人消化杂志》2006年第28期2747-2752,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30470777~~

摘　　要：目的:探讨阿斯匹林和选择性COX-2抑制剂塞莱昔布对体外培养的幽门螺杆菌(H pylori)生长、毒力因子及外膜蛋白的影响．方法:不同浓度的阿司匹林及塞莱昔布与H pylori共同培养,以活菌计数、分光光度计法检测H pylori的生长状态,分光光度计检测A 560nm值判断尿素酶活性,Hela细胞空泡变性实验和中性红吸收试验检测空泡毒素的活性,以SDS-PAGE电泳检测H pylori外膜蛋白的变化．结果:阿司匹林及塞莱昔布可以抑制H pylori的生长,此过程为剂量依赖性效应．阿司匹林0．5 mmol／L及塞莱昔布0．01 mmol／L时与DMSO对照相比H pylori24 h和48 h的菌落计数开始降低,随着两药剂量的加大菌落计数降低得更加明显,阿司匹林2．0 mmol/L和塞莱昔布0．04 mmol／L时H pylori被完全杀灭．阿司匹林及塞莱昔布可剂量依赖性的抑制H pylori的尿素酶活性及空泡毒素的活性．在对H pylori外膜蛋白的研究中发现,NSAIDs可能对H pylori的某种外膜蛋白表达有影响．结论:阿司匹林和塞莱昔布可抑制H pylori的生长、毒力因子的活性,并可能改变H pylori外膜蛋白的表达．AIM： To investigate the possible effects of aspirin and celecoxib on the growth, virulence factors and outer membrane protein of H pylori. METHODS： Two standard strains of H pylori were treated with a series concentration of aspirin or celecoxib （a specific inhibitor of cyclooxygenase 2） and were incubated in brucella broth under microaerobic condition for 24 and 48 h. The growth and urease activity were assessed by viable colony counting and spectrophotometry. After 48-hour treatment, the supernatants were incubated with Hela cells to estimate vacuolating cytotoxin activity. The outer membrane protein of H pylori was analyzed by SDS-PAGE. RESULTS： Aspirin or celecoxib inhibited the growth of H pylori in a dose-dependent manner with the lowest inhibitory concentrations of 0.5 or 0.01 mmol/L, and the lethal concentrations of 2.0 and 0.04 mmol/L, respectively. Aspirin and celecoxib inhibited the urease activity and vacuolating cytotoxin activity of H pylori. The inhibitory effect of celecoxib on H pylori was superior to that of aspirin. Both aspirin and celecoxib had some effects on the expression of the outer membrane protein. CONCLUSION： Aspirin or celecoxib can inhibit the growth, urease activity and vacuolating cytotoxin activity of H pylori in vitro significantly. Both aspirin and celecoxib may have some effects on the outer membrane protein expression of H pylori.

关 键 词：幽门螺杆菌 非甾体类消炎药 阿司匹林 塞莱昔布 

分 类 号：R573[医药卫生—消化系统]