结直肠癌中PTEN的缺失表达及临床意义  

Down-regulated expression of PTEN in colorectal cancer and its clinical significance

作　　者：李季[1] 田素礼[1] 李巍[1] 李福蕴 

机构地区：[1]哈尔滨医科大学附属二院普外二科,黑龙江省哈尔滨市150086 [2]安达市中医院,黑龙江省安达市151400

出　　处：《世界华人消化杂志》2006年第28期2771-2775,共5页World Chinese Journal of Digestology

摘　　要：目的:研究PTEN在结直肠癌中的表达及其与结直肠癌病理特征的关系．方法:应用免疫组化SP法分别检测65例癌组织和癌旁组织及13例腺瘤组织PTEN蛋白的表达．结果:PTEN主要在细胞核或细胞质中表达,在结直肠癌组织中阳性表达率显著低于癌旁组织阳性表达率(56．92% vs 86．15％,P<0．01)．中、高分化腺癌阳性表达率显著高于低、未分化腺癌阳性表达率(75．76% vs 37．50％, P<0．01)．Dukes A、B期阳性表达率显著高于Dukes C、D期阳性表达率(73．33％vs 42．86％, P<0．05)．此外,PTEN的表达与淋巴转移有关(P<0．01),而与性别、年龄、同期的肿瘤大小无关．结论:PTEN在结直肠癌组织中的表达下调,其表达水平可作为反映结直肠癌进展和预后的生物学指标之一．AIM： To study the expression of PTEN in colorectal carcinoma and its correlation with the clinicopathological features. METHODS： Immunohistochemical SP technique was used to determine the expression of PTEN protein in 65 cases of colorectal carcinoma and their corresponding adjacent tissues, 13 cases of adenoma tissues, and 20 cases of normal colorectal tissues. RESULTS： The expression of PTEN protein was mainly located in the nucleus and cytoplasm. The expression of PTEN protein was significantly lower in human colorectal cancer tissues than that in the corresponding adjacent tissues ones （56.92% vs 86.15%, P 〈 0.01）, and it was also markedly lower in the lowly- and non-differentiated adenoma than that in the highly-and moderately-differentiated one （37.50% vs 75.76%, P 〈 0.01）. The expression of PTEN protein in Dukes A and B stages were significantly higher than that in in Dukes C and D stages （73.33% vs 42.86%, P 〈 0.05）. In addition, PTEN expression was correlated with lymph node metastasis （2 = 7.448, P 〈 0.01）, but not with the sex, tumor sizes of patients. CONCLUSION： The expression of PTEN is down-regulated in colorectal carcinoma, and it may be one of the molecular and biological indicators in reflecting the progression and prognosis of colorectal cancer.

关 键 词：结直肠癌 癌旁组织 PIEN基因 转移 

分 类 号：R735.3[医药卫生—肿瘤]