胰腺导管腺癌组织中WT1,IGF-IR表达与细胞凋亡的关系  

Relationship between expression of Wilms' tumor gene,insulin-like growth factorⅠreceptor and cell apoptosis in human pancreatic duct adenocarcinoma

作　　者：龚勇[1] 赵秋[1] 杨芳[1] 王渝[1] 马松林[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科,湖北省武汉市430030

出　　处：《世界华人消化杂志》2006年第28期2810-2814,共5页World Chinese Journal of Digestology

摘　　要：目的:研究胰腺导管腺癌组织中WT1,IGF-IR的表达与细胞凋亡关系．方法:应用免疫组化技术检测WT1,IGF-IR在49例胰腺导管腺癌及15例正常胰腺组织中的表达,并应用TUNEL法检测细胞凋亡,计算凋亡指数(AI)．结果:WT1,IGF-IR在正常胰腺组织中的阳性表达率分别为26．67％(4／15)、40．00％(6／15);在胰腺导管腺癌组织中的阳性表达率分别为71．43％(35／49)、77．55％(38／49),两者在癌组织中的表达分别明显高于其在正常胰腺组织中的表达(P<0．05),且在癌组织中的表达呈正相关(r=0．385,P<0．05)．正常胰腺组织及癌组织中的AI分别为0．41±0．13、5．93±4．18,两者比较有显著性差异(P<0．05),癌组织中AI随组织分化程度的升高而升高．IGF-IR表达阳性组的AI显著低于阴性组(4．11±3．68 vs 12．21±5．67,P<0．01)．结论:胰腺导管腺癌组织中IGF-IR的高表达抑制细胞凋亡,WT1,IGF-IR的高表达以及细胞凋亡的减少可能在胰腺导管腺癌的发生发展中起重要作用．AIM： To investigate the roles of Wilms＇ tumor gene （WT1）, insulin-like growth factor Ⅰ receptor expression （IGF-IR） and cell apoptosis as well as their correlations in human pancreatic duct adenocarcinoma. METHODS： Immunohistochemical analysis was used to detect the expression of WT1 and IGF- IR in human pancreatic duct adenocarcinoma （n = 49） and normal pancreatic tissues （n = 15）. The cell apoptosis of pancreatic duct adenocarcinoma was estimated by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） method and apoptosis index （AI） was calculated. RESULTS： In normal pancreatic tissues, the positive rates of WT1, IGF-IR were 26.67% （4/15）, 40.00% （6/15）, respectively, and in pancreatic duct adenocarcinoma their positive rates were 71.43% （35/49）, 77.55% （38/49）, respectively. There were significant differences between cancer and normal tissues （P 〈 0.05）. A positive relationship existed between the expression of WT1 and IGR-IR in pancreatic duct adenocarcinoma （r = 0.385, P 〈 0.05）. The values of AI were 0.41 ± 0.13 and 5.93 ±4.18 in the normal tissues and adenocarcinoma, respectively, and there was also a significant difference between them （P 〈 0.05）. The value of AI was increased with the rising of tumor differentiation degree in adenocarcinoma, and it was lower in the IGF-IR-positive group than that in the negative group （4.11± 3.68 vs 12.21 ±5.67, P 〈 0.01）. CONCLUSION： The apoptosis is inhibited in pancreatic duct adenocarcinoma, in which the over-expression of WT1 and IGF-IR may play crucial roles.

关 键 词：Wilms肿瘤基因 胰岛素样生长因子Ⅰ受体 胰腺导管腺癌 免疫组织化学 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]