4-1BB信号对小鼠树突状细胞表面TLR4表达的调节  

4-1BB-mediated regulation of Toll-like receptor 4 expression on nawine dendritic cells

作　　者：马倩茹[1] 张烽[1] 周桓[1] 於葛华[1] 张学光 顾宗江[2] 

机构地区：[1]苏州大学医学院免疫学教研室,215007 [2]江苏省临床免疫学重点实验室

出　　处：《中华微生物学和免疫学杂志》2006年第10期867-870,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨4-1BB(CD137)激发型单克隆抗体2A对小鼠骨髓来源的树突状细胞(DC)表面TLR4表达的调节。方法用不同剂量2A、LPS以及2A与LPS联合,或用LPS预刺激后再加入2A,以流式细胞术检测这些处理因素作用下DC表面TLR4表达情况。结果LPS可下调DC TLR4的表达,下调作用可维持24h,而2A可使DC TLR4的表达上调,上调作用可维持12h,并可拮抗LPS对TLR4的下调作用。用LPS预处理DC后再加入2A,也可拮抗LPS的下调作用。结论4-1BB信号可以上调DC表面TLR4的表达。Objective To study regulatory effect of agonist 4-1BB （CD137） monoclonal antibody 2A on TLR4 expression in murine dendritic cells （DC）. Methods Flow cytometry was used to investigate the TLR4 expression on DC in following conditions： different doses of 2A, LPS, 2A combined with LPS, or addition of 2A after prestimulation of DC by LPS. Results LPS could down-regulate TLR4 expression on DC, and the down-regulatory effect of LPS was maintained for 24 h. On the contrary, 2A could up-regulate TLR4 expression on DC, and this effect was maintained for 12 h. Moreover, 2A inhibited the down-regulatory effect of LPS on TLR4 expression. When DC were stimulated by 2A after LPS prestimulation , 2A still up-regulated TLR 4 expression on DC. Conclusion 4-1BB signal can up-regulate the expression of TLR4 on dendritic cells.

关 键 词：树突状细胞 TLR4 4-1BB 

分 类 号：R392[医药卫生—免疫学]