基底细胞癌和鳞状细胞癌中转化生长因子βⅠ型和Ⅱ型受体表达  

Down-regulation of TGFβreceptor expression in human basal cell carcinoma and squamous cell carcinoma

作　　者：李颖[1] 何威[1] 阎国富[1] 何云志[1] 黄海[1] 姚磊[2] 林自华[1] 吴军[1] 

机构地区：[1]第三军医大学新桥医院皮肤科,重庆400037 [2]第三军医大学新桥医院检验科,重庆400037

出　　处：《中华皮肤科杂志》2006年第11期621-624,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助项目(30371298)

摘　　要：目的探讨转化生长因子β(TGFβ)受体在基底细胞癌和鳞状细胞癌中表达水平的变化及其意义。方法采用实时定量PCR和SP免疫组化技术分别检测基底细胞癌、鳞状细胞癌及正常人对照皮肤中Ⅰ型和Ⅱ型TGFβ受体(TGFβRⅠ,TGFβRⅡ)mRNA和蛋白的表达。结果对18例基底细胞癌、24例鳞状细胞癌患者的皮损及正常人对照皮肤的研究显示,基底细胞癌和鳞状细胞癌皮损TGFβRⅠ和TGFβRⅡ的mRNA表达水平均显著低于正常人对照皮肤。免疫组化试验结果显示:TGFβRⅠ染色强度在基底细胞癌组、鳞状细胞癌组较正常人对照皮肤组显著降低(P<0．001);TGFβRⅡ在二组表皮肿瘤与正常人对照皮肤组问表达差异也有统计学意义(P<0．01)。结论基底细胞癌和鳞状细胞癌中TGFβ受体表达下调可能有助于这些上皮细胞起源的表皮肿瘤的形成。Objective To investigate the expression and significance of transforming growth factor beta receptors （TGFβR Ⅰ ,TGFβR Ⅱ ） in basal cell carcinoma and squamous cell carcinoma. Methods The expression of TGFβR Ⅰ and TGFβ R Ⅱ were assessed by quantitative real-time PCR and streptavidin-peroxidase （SP） immunohistochemical techniques in specimens of basal cell carcinoma, squamous cell carcinoma and normal control skin. Results Eighteen patients with basal cell carcinoma and twenty-four with squamous cell carcinoma were enrolled in the study. The expression levels of TGFβ RⅠ and R Ⅱ mRNA were significantly down-regulated in basal cell carcinoma and squamous cell carcinoma in comparison with those in control skin specimens （ P 〈 0.001 ）. The intensity of positive immunostaining for TGF β R Ⅰ and TGF β R Ⅱ were also significantly down-regulated in basal cell carcinoma and squamous cell carcinoma in comparison with those in normal skin （ P 〈 0.01 ）. Conclusions Down-regulation of TGF β receptor expression may interfere with TGF β signaling transduction pathway, and contribute to the formation of basal cell carcinoma and squamous cell carcinoma.

关 键 词：癌 基底细胞 癌 鳞状细胞 转化生长因子Β 受体 转化生长因子β 

分 类 号：R730.2[医药卫生—肿瘤]