DAB诱发大白鼠肝癌过程中细胞质膜上酶变化的观察  

Observation on the Changes of Enzyme Activities in Plasma Membrane from Rat LiversDuring Hepatocarcinogenes is Induced by DAB

作　　者：黄文心[1] 徐晓利[1] 马涧泉[1] 

机构地区：[1]中山医科大学生物化学教研室

出　　处：《齐鲁肿瘤杂志》1996年第2期97-99,共3页

摘　　要：本实验以二甲氨基偶氮苯（ＤＡＢ）诱发大白鼠肝癌的动物模型为材料，观察了在诱癌过程中和肝癌形成后大白鼠肝细胞质膜上几种酶活性的变化。用不连续蔗糖密度梯度离心法制备肝细胞质膜，用分光光度法对酶活性进行定量测定。实验结果表明，在诱癌过程中，肝细胞质膜上５＇－ＡＭＰ酶活性下降，γ－ＧＴ_(ａｓｅ)酶活性显著升高。γ－ＧＴ_(ａｓｅ)酶活性升高幅度与病理变化正相关，并且在诱癌早期就能表现出来。The authors had observed the Changes of enzymic a ct ivities in plasma membranes during DAB induced rat hepatocarcinogenesis.The plamsa membrane of liver cell were taken by discontinuous sucrose density gradient centrifugation method.The enzymic activities were determined quatitively by spectrophotometry.The results revealed that the 5'-AMPase ac tivities decrease during carcinogenesis,whereas the γ-GTase ativities showed a great increase which were correlated positively with the pathologic change of the hepatic cell.

关 键 词：肝癌 谷氨酰转肽酶 腺苷酸酶 

分 类 号：R735.702[医药卫生—肿瘤]