胶质瘤p53、p16、bcl-2、c-fos蛋白表达和细胞凋亡  

Expression of p53,p16,bcl-2,c-fos protein and cell apoptosis in 150 cases of cerebral glioma

作　　者：吴礼高[1] 何杰[2] 王朝夫[3] 吴强[1] 龚晓萌[4] 

机构地区：[1]安徽医科大学病理学教研室,安徽合肥230032 [2]东南大学附属中大医院病理科,江苏南京210009 [3]复旦大学附属肿瘤医院病理科,上海200032 [4]蚌埠医学院病理学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2006年第6期572-574,F0004,共4页Journal of Bengbu Medical College

基　　金：安徽省自然科学基金资助项目(00044302)

摘　　要：目的:探讨p53、p16、bcl-2、c-fos基因在胶质瘤的表达水平和细胞凋亡与胶质瘤的发生、发展的关系。方法:采用免疫组织化学和原位末端标记(ISEL)技术,对不同组织学级别150例胶质瘤石蜡标本进行基因蛋白和细胞凋亡的检测。结果:150例胶质瘤中p53、p16、bcl-2、c-fos蛋白的阳性率分别为50.00%、38.67%、61.33%、46.67%;p53、p16、bcl-2蛋白表达差异在各组间均有统计学意义(P<0.005),c-fos蛋白表达差异无统计学意义(P>0.05);随胶质瘤级别升高,bcl-2蛋白表达水平增高,瘤细胞凋亡减少,但差异无显著性(P>0.05)。结论:p53、p16、bcl-2、c-fos基因的异常表达在胶质瘤的发生、发展过程中起重要作用。p53、p16、bcl-2、c-fos基因的协同作用促使细胞凋亡机制障碍,细胞增殖和凋亡失控。p53、p16、bcl-2、c-fos基因蛋白的检测对判断预后、指导临床基因治疗有重要意义。Objective ：To investgate the relationship between p53, p16, bcl-2 ,c-fos gene expression level and cell apoptosis in cerebral glioma with the development and progression, Methods：The expression of p53, p16, bcl-2, c-fos protein was detected in 150 cases of different grade cerebral glioma by immunohistochemistry and cell apoptosis by using in situ end labeling（ISEL） technique. Results ：The positive expression rate of p53 ,p16, bcl-2, c-fos protein in 150 cases of cerebral glioma was 56.67% ,38.67% ,61.37% ,46.67% , respectively;There was significant difference of the expression of p53, p16, bcl-2 protein among I - Wgrade glioma （ P 〈 0. 005 ） , there was no significant difference of the expression of c-fos protein （ P 〉 0. 05 ）. With the higher of the positive intensity of bcl-2 protein, ceil apoptosis was decrease in the tumor, but there was no significant difference of the ceil apoptosis （ P 〉 0.05 ）. Conclusions： The abnormal expression of p53, p16, bcl-2, c-fos protein played an important role in the development and progression of cerebral glioma. The cooperation of the p53, p16, bcl-2, c-fos protein may cause disorder of ceil apoptosis, and unbalance of cell proliferation and apoptosis. Then the detection of p53, p16 ,bcl-2, c-fos protein may play an important role in prognosis and clinic gene therapy.

关 键 词：脑肿瘤 胶质瘤 癌基因蛋白类 凋亡 免疫组织化学 原位末端标记 

分 类 号：R739.41[医药卫生—肿瘤] R730.264[医药卫生—临床医学]