新辅助化疗对乳腺癌细胞凋亡和增殖的影响  

Influence of neoadjuvant chemotherapy on apoptosis and proliferation in patients with breast caner

作　　者：付晓艳 秦锡虎[2] 陈同珏[3] 谈敏[3] 

机构地区：[1]江苏省常州市妇幼保健院乳腺科,213001 [2]江苏省常州市第一人民医院肝胆外科,213001 [3]江苏省常州市第一人民医院病理科,213001

出　　处：《蚌埠医学院学报》2006年第6期624-626,F0003,共4页Journal of Bengbu Medical College

摘　　要：目的:探讨新辅助化疗对乳腺癌肿瘤细胞的凋亡和增殖的影响。方法:应用末端脱氧核苷酸转移酶介导dUTP缺口末端标记法(TdT-mediated dUTP-biotin nick end labeling,TUNEL)及免疫组化的S-P法,分别检测新辅助化疗组和对照组乳腺癌组织中肿瘤细胞的凋亡指数(AI)和乳腺癌肿瘤组织的Ki-67抗原表达。结果:新辅助化疗组肿瘤细胞AI均数为9.26%,比对照组肿瘤细胞AI均数3.36%明显增高(P<0.01)。新辅助化疗组肿瘤细胞Ki-67抗原过表达率为15.63%,比对照组Ki-67抗原过表达率50.0%低(P<0.05)。结论:新辅助化疗能诱导人体乳腺癌肿瘤细胞发生凋亡,并能抑制其增殖。Objective：To study the influence of neoadjuvant chemotherapy on tumor cell apoptosis and proliferation in patients with breast cancer. Methods：The apoptosis index（AI） of tumor cells in the neoadjuvant group and control group was assayed by TdTmediated dUTP-biotin nick end labeling（TUNEL） , and the Ki-67 antigen was examined by immunohistochemical method. Results：The AI of tumor ceils was 9.26% in the neoadjuvant group and 3.36% in the control group. The difference was significant（ P 〈0.01 ）. The overexpression rate of Ki-67 antigen in the neoadjuvant group was 15.63% ,which was 50.0% lower than that in the control group（ P 〈 0.05）. Conclusions： Neoadjuvant chemotherapy can induce tumor cell apoptosis and inhibit tumor cell proliferation in patients with breast cancer.

关 键 词：乳腺肿瘤 肿瘤/药物疗法 细胞凋亡 KI-67抗原 

分 类 号：R737.5[医药卫生—肿瘤] R730.53[医药卫生—临床医学]