光谱核型分析技术——一种新的染色体核型分析方法  

SPECTRAL KARYOTYPING——AN NEW METHOD FOR CHROMOSOME ANALYSIS

作　　者：周丽英[1] 钱建新[1] 郭晓葵[1] 戴宏[1] 刘玉龙[1] 周剑影[1] 庄志祥[1] 姜忠[1] 

机构地区：[1]苏州大学附属第二医院,江苏苏州215004

出　　处：《辐射防护》2006年第6期371-374,380,共5页Radiation Protection

摘　　要：光谱核型分析(spectral karyotyping,SKY)技术可揭示G、R、Q带通常无法检测到的染色体结构上的细微变异,已成为染色体核型分析的一种精确、灵敏和可靠的检测手段。它具有高度的敏感性和特异性,使细胞遗传学向分子领域发展,并在临床、辐射损伤和科研等工作中得到广泛应用。SKY技术一次成像可同时区分24条染色体,例如结构复杂的易位、缺失、扩增、重排、双着丝粒、等臂及标记染色体,检测稳定性染色体畸变等,并能准确推算生物剂量曲线。SKY技术检测染色体异常可作为早期诊断、治疗监测和随访过程中的有效指标,为相关基因的克隆及癌症的发病机制的研究提供了更先进和有效的方法。本文主要介绍这一技术的方法和原理。Spectral Karyotyping（SKY） can reveal fine changes in Chromosome structure which could not be detected by G,R, Q banding before, has become an accurate, sensitive and reliable method for karyotyping, promoted the development of cell genetics to molecular level and has been used in medicine and radiological injury research. It also has the ability of analyzing 24 chromosomes on its once test run and, find implicated structure of chromosome changes, such as metathesis, depletion, amplification, rearrangement, dikinetochore, equiarm and maker-body, detect the abnormal change of stable Chromosome and calculate the bio-dose curve; The abnormal Chromosome detected by SKY can be adopted as early diagnosis, effective indexes of minor remaining changes for use of monitor of treatment and in the duration of follow up. This technique provides us a more advanced and effective method for relative gene cloning and the study of pathological mechanism of cancer.

关 键 词：光谱核型分析 荧光原位杂交 染色体 

分 类 号：Q343.2-33[生物学—遗传学]