丹皮多糖分子的电子显微镜观察  

TEM observation of Polysaccharide from Root of Paeonia suffruticosa Andr

作　　者：戴玲[1] 沈业寿[1] 庄永龙[2] 赵帜平[1] 廖洪梅[1] 

机构地区：[1]安徽大学生命科学学院安徽省生态工程与生物技术重点实验室,安徽合肥230039 [2]安徽大学现代实验技术中心,安徽合肥230039

出　　处：《电子显微学报》2006年第5期405-407,共3页Journal of Chinese Electron Microscopy Society

基　　金：安徽省自然科学基金资助项目(03043301);安徽大学211工程学术创新团队资助项目

摘　　要：利用透射电镜对丹皮多糖分子(PSM2b-A)的显微结构进行了研究。观察到PSM2b-A在水溶液中呈球状或圈状的形貌,这些球状和圈状的结构可进一步连接或侧向聚集成链状或网状结构。表明在稀水溶液中,多糖分子倾向于蜷曲,且可通过氢键和其他弱作用力形成各种分子聚集形态。The uhrastructuro of polysaccharide isolated from the root of Paeonia suffruticosa Andr named as PSM2b-A was examined by transmission electron microscopy. PSM2b-A samples in aqueous solution were heated at 45℃ for 20 minutes before dipping to the copper grids following negative staining by uranyl acetate after air-dried. Coiled balls or circles, extended chains, network-like structures have been observed. It is presumed that polysaccharide molecules of PSM2b-A in aqueous solution are likely to coil into floppy or compact globular structures which may assemble into aggregates by means of hydrogen bonds, leading to a variety of morphology. The TEM images provide useful insight into the conformations and interactions of polysaccharide molecules.

关 键 词：多糖 丹皮 电子显微镜 

分 类 号：R284[医药卫生—中药学]