利用特异性Y染色体研究肝脏卵圆细胞在原发性肝癌发生中的作用  

Investigation of the role of hepatic oval cell in primary hepatocarcinogenesis using specific Y chromosome

作　　者：方驰华[1] 陈铁军[1] 刘胜军[1] 

机构地区：[1]南方医科大学珠江医院肝胆外科

出　　处：《中华外科杂志》2006年第21期1501-1504,共4页Chinese Journal of Surgery

基　　金：广东省自然科学基金资助项目(2002020097)

摘　　要：目的利用Y染色体细胞的特异性来研究肝脏卵圆细胞分化为肝癌细胞的直接证据,从而为卵圆细胞源性的研究及将来卵圆细胞的临床应用提供理论依据。方法30只雄性Wistar大鼠喂含0·06%的3-甲基-4-二甲基偶氮苯(DAB)的饲料4周,建立动物模型。将雌性Wistar大鼠40只随机平分成对照组和实验组,对照组不接种提取的雄性Wistar大鼠卵圆细胞悬液,连续喂含DAB的饲料14周。实验组肝脏包膜下接种悬液,连续喂DAB饲料14周,以促进肝肿瘤的生成。设计引物片断,14周后提取两组大鼠肝肿瘤组织的基因组DNA,应用PCR扩增,对产物进行电泳分析。结果14周后40只雌性大鼠均见肝脏肿瘤生成,实验组电泳后均见与设计片断长度相符的电泳阳性条带,对照组则未见阳性条带。结论大鼠的卵圆细胞可分化为肝癌细胞,为卵圆细胞源性肝癌细胞的假说提供实验依据。Objective To determine the role of hepatic oval cell in primary hepatocarcinogenesis using specific Y chromosome. Methods The model of hepatic oval cell proliferation was established by feeding 30 male Wistar rats with 0. 06% 3＇3-diaminobenzidine （DAB） for 4 weeks. Another 40 female Wistar rats were equally randomized into two groups： control group and experimental group. Experimental group was inoculated with oval cells suspension from the prepared male rats under the hepatic amicula and fed with DAB for 14 weeks continuously to promote hepatocarcinogenesis. Control group was fed with DAB for 14 weeks. After primer fragments were worked out on the male rats＇ SRY genes using the software of primer design, DNAs were extracted from the hepatic carcinomas of the female rats of the two groups and then amplified with PCR. Electrophoretic analysis was performed on the products. Results After 14 weeks, primary hepatocarcinogenesis was found in the livers of all the 40 female rats. Electrophoresis showed the positive straps from the experimental group with the same length to the designed segments, whereas no positive straps were seen in the control group. Conclusions The hepatic oval cells can differentiate into hepatic cancer cells, which provides the evidence that the hepatocellular carcinoma has its source from the hepatic oval cells.

关 键 词：肝肿瘤 Y染色体 肝卵圆细胞 

分 类 号：R735.7[医药卫生—肿瘤]