层黏素受体下调对蛋白水解酶MMP-2、MMP-9、u-PA蛋白表达的影响  

Effect of down-regulated laminin receptor of cell line QBC_(939) on protein expressions of MMP-2,MMP-9 and u-PA in human cholangiocarcinoma cells

作　　者：李大江[1] 陈健[1] 陈长宏[1] 王曙光[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,中国人民解放军西南肝胆外科医院,重庆400038

出　　处：《消化外科》2006年第6期455-458,共4页Journal of Digestive Surgery

基　　金：国家自然科学基金资助项目(No.39870830)

摘　　要：目的研究层黏素受体(LNR)反义硫代磷酸寡核苷酸片段(AsODN)对人胆管癌QBC939细胞基质蛋白水解酶-2(MMP-2)、基质蛋白水解酶-9(MMP-9)以及尿激酶型纤维蛋白溶解酶原激酶(u-PA)蛋白表达的影响。方法应用Western blotting分析浓度12μmol/L的LNR AsODN作用48h后,QBC939细胞MMP-2、MMP-9以及u-PA蛋白表达水平。结果LNR AsODN能明显抑制人胆管癌细胞表达MMP-2、MMP-9以及u-PA,与对照组相比,MMP-2、MMP-9、u-PA蛋白表达下降分别为21.8%、16.5%和9.0%(P<0.05)。结论胆管癌细胞QBC939蛋白水解酶MMP-2、MMP-9以及u-PA基因表达水平与层黏素受体的表达水平明显相关,LNR AsODN表达能够使胆管癌细胞蛋白水解酶基因表达下降,提示层黏素受体在介导胆管癌转移中发挥重要作用。Objective To investigate the effect of antisense oligodeoxynucleotide （AsODN） of laminin receptor （LNR） on expressions of matrix metalloproteinases-2 （MMP-2）, MMP-9 and u-PA in human cholangiocarcinoma cells. Methods Protein expressions of MMP-2, MMP-9 and u-PA of cell line QBC939 were analyzed by using Western blotting after lipofectAMINE and AsODN phosphorothioate of LNR at concentrations of 12 μmol/L were cultured for 48 hours. Results Western blotting showed that AsODN could significantly down-regulate protein expressions of MMP-2, MMP-9 and u-Pap, with decrease rate of 21.8 %, 16.5 % and 9.0 % respectively （ P 〈0.05） in comparison with the control group. Conclusions Gene expressions of MMP-2, MMP-9 and u-PA are markedly related to expression of laminin receptor in cholangioeareinoma cells and can be decreased by expression of LNR AsODN, suggesting an important role of laminin receptor in the invasion and metastasis of cholangiocarcinoma.

关 键 词：受体 层黏素 蛋白水解酶 胆管肿瘤 细胞系 寡核苷酸类 反义 

分 类 号：R735.8[医药卫生—肿瘤]