腹腔注射脂多糖对大鼠海马tau蛋白磷酸化的影响  

Effect of intraperitoneal injection of LPS on tau hyperphosphorylation in rat hippocampus

作　　者：刘英华[1] 尹君[1] 王建枝[1] 

机构地区：[1]华中科技大学同济医学院病理生理学系,湖北武汉430030

出　　处：《中国病理生理杂志》2006年第11期2086-2089,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30430270No.30328007)

摘　　要：目的:探讨脂多糖(LPS)对大鼠海马tau蛋白异常磷酸化的影响。方法:腹腔注射不同剂量的LPS后不同时点取材,采用W estern b lotting技术检测海马tau蛋白异常磷酸化情况。结果:1.腹腔注射LPS后均出现了tau蛋白表达总量增加;2.注射低剂量LPS(200μg/kg BW)后8 h、16 h,tau蛋白Ser396/404位点发生异常过度磷酸化,而64 h则发生去磷酸化;Ser199/202位点在各时点均发生去磷酸化;3.注射高剂量LPS(2 mg/kg BW)时,tau蛋白Ser396/404、Ser199/202及Ser422位点的磷酸化状态未发生改变,而在6 h和24 h,Ser214位点出现了过度磷酸化。结论:不同剂量的LPS腹腔注射可影响大鼠海马tau蛋白不同位点的磷酸化水平。AIM： To investigate the effect of LPS on tan hyper- phosphorylation in rat hippocampus. METHODS： After intraperitoneal injection of different doses of LPS, the changes of tau phosphorylation in rat hippocampus at different time points were detected. RESULTS： 1. Intraperitoneal injection of LPS resulted in a significant increase in total level of tan. 2. tau was hyperphosphorylated at Ser396/404 at 8 h and 16 h, then dephosphorylated at 64 h after low dose injection of LPS （200 μg/kg BW） , while tan was dephosphorylated at Ser199/202 by the same treatment. 3. The phosphorylation status of tau at Ser396/404, Ser199/202 and Ser422 was not changed obviously with higher dose injection of LPS （2 mg/kg BW）, and at this condition, hyperphosphorylation of tan at Ser214 was detected after 6 h and 24 h of the injection. CONCLUSION： Intraperitoneal injection of LPS influences tan phosphorylation in rat hippocampus at some sites seen in the brain of Alzheimer disease, implying that the involvement of inflammation is in the pathogenesis of Alzheimer＇ s disease.

关 键 词：阿尔茨海默病 脂多糖类 炎症 TAU蛋白质类 海马 

分 类 号：R745.7[医药卫生—神经病学与精神病学]