血红素氧合酶-1对心肌相对缺血再灌注损伤的延迟保护作用(英文)  

Delayed protection of HO-1 in the exercise preconditioning from the myocardial relative ischemic reperfusion injury

作　　者：翟庆峰[1] 刘洪涛[1] 马强[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国病理生理杂志》2006年第11期2127-2131,共5页Chinese Journal of Pathophysiology

摘　　要：目的:探讨血红素氧合酶-1(HO-1)在运动预适应(EP)中对大鼠心肌相对缺血再灌注(rI/R)损伤的延迟保护作用及机制。方法:40只W istar大鼠随机分为5组:正常对照组(CN)、相对缺血再灌注组(IR)、运动预适应+相对缺血再灌注组(EI)、Hem in(HO-1诱导剂)+相对缺血再灌注组(HE)和运动预适应+ZnPP(HO-1抑制剂)+相对缺血再灌注组(EZ)。测定大鼠再灌注期心率脉压乘积(PRP)、冠脉流出液MDA含量、HO-1活性等。结果:心肌HO-1活性:EI组和HE组较IR组显著升高,EZ组则显著降低。EZ组较EI组显著降低,EI组和HE组间亦有显著差异。再灌注后60 m in时点PRP恢复率:EI组较IR组显著增高;IR组与HE组未见显著差异,但30 m in时点HE组显著增高;EZ组较EI组显著降低。冠脉流出液MDA含量:EI组、EZ组和HE组MDA含量较IR组皆显著降低;EZ组较EI组显著升高。结论:EP可以诱导HO-1合成,进而通过HO-1对24 h后发生的rI/R损伤产生延迟保护作用。AIM ： To explore the delayed protection of heine oxygenase - 1 （ HO - 1 ） in the exercise preconditioning （EP） from the myocardial relative ischemia reperfusion injury （rl/R）. METHODS： 40 Wistar Rats were divided into 5 groups randomly： control group （ CN）, rI/R group （ IR）, EP ＋ rI/R group （ EI）, HO - 1 inductor hemin ＋ rI/R group （HE） and HO - 1 inhibitor ZnPP ＋ EP ＋ rI/R group （EZ）. The following indexes were detected, including the HO - 1 activity in myocardium, the cardiac function parameter - pressure - rate product （ heart rate x left ventricular developed pressure, PRP） and the content of MDA in coronary effluent. RESULTS： After myocardial rI/R, HO - 1 activity increased significantly. Moreover, EP or HO - 1 inductor could enhance this effect manifestly. Nevertheless, when the HO - 1 inhibitor was administered before EP,HO - 1 activity decreased. In addition, there was no distinct difference in the HO - 1 activity between EI group and HE group. At the 30 min point of reperfusion, the PRP recovery rate of EI group was higher clearly than that of IR group. However, there was reverse effect between the EZ group and the EI group. The MDA in coronary effluent of EI group, EZ group and HE group were lower obviously than that of IR group and there was significant difference between EI group and EZ group. CONCLUSION： EP could protect the heart from the rI/R injury occurring 24 hours later, which might be performed through activating the HO - 1.

关 键 词：血红素氧合酶 运动预适应 延迟预适应 心肌再灌注损伤 

分 类 号：R363[医药卫生—病理学]