姜黄素对前列腺癌细胞LNCaP增殖的影响  

Effect of curcumin on the proliferation and apoptosis in LNCap cells

作　　者：杨磊[1] 张莲英[1] 陈蔚文[1] 胡晓燕[1] 张建业[1] 崔福爱[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,山东济南250012

出　　处：《中国病理生理杂志》2006年第11期2194-2197,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30171026);山东省中医药局立项项目

摘　　要：目的:姜黄素对前列腺癌细胞株LNCap增殖和凋亡的影响。方法:用不同剂量的姜黄素分别处理LNCap细胞,显微镜下观察细胞形态;MTT法检测细胞生长情况;流式细胞技术(FCM)检测细胞凋亡率;然后检测姜黄素处理LNCap细胞后培养液中总前列腺特异抗原(PSA)的变化,并用免疫印迹W estern b lotting技术检测雄激素受体(AR)的表达。结果:姜黄素能够抑制前列腺癌细胞LNCap的增殖和生长,40μmol/L作用24 h最强,细胞存活率为对照的40%;姜黄素诱导LNCap细胞凋亡,细胞形态呈凋亡特征,且40μmol/L作用最强,凋亡率为9.23%;姜黄素抑制LNCap细胞PSA表达,且40μmol/L姜黄素处理细胞24 h对PSA表达的抑制作用最强,PSA含量仅为对照的20%;W estern b lotting检测结果显示姜黄素抑制AR的表达,并且对AR表达的抑制程度依赖于姜黄素的浓度。结论:姜黄素抑制LNCap细胞增殖,诱导细胞凋亡,且表现出时间和剂量依赖性。姜黄素抑制LNCap细胞PSA与AR受体的表达。AIM ： To observe the effect of curcumin on the proliferation and apoptosis of prostate cancer cell line LNCap. METHODS： LNCap cells were treated with different doses （ 10 μmol/L, 20 μmol/L, 30 μmol/L, 40 μmol/L） of curcumin and its effects were analyzed in cell growth and apoptosis by microscope, MTT colorimetric assay and flow cytometry. The expression＇of prostate specific antigen （PSA） was measured by AXSYMTM system -chemical luciferase methods and expression of androgen receptor （AR） was detected by Western blotting. RESULTS： The results showed that curcumin inhibited the proliferation of LNCap cells. The cell growth was inhibited by curcumin in a dose dependent manner and the optimal dose and time was 40 μmol/L, 24 h. Curcumin induced apoptosis in LNCap cells, the most dramatic dose was 40 μmol/L curcumin, at this dose the apoptosis rate was 9.23%. Curcumin inhibited the expression of PSA in LNCap cells and the most dramatic dose and time was 40 μmol/L, 24 h. The PSA in this group was 20% of the control group. Curcumin inhibited the expression of AR on prostate cancer cells. CONCLUSION： Curcumin decreases proliferation and induces apoptosis in LNCap cells in a dose -dependent manner. Curcumin also inhibites the expression of PSA and AR on LNCap cells.

关 键 词：前列腺肿瘤 姜黄素 细胞凋亡 LNCAP细胞 

分 类 号：R737.25[医药卫生—肿瘤]