胃癌患者PBMCs中CD4^+ CD25^+ T细胞体外增殖及对CD4^+ CD25^- T细胞增殖的影响  

Proliferation of CD4^+ CD25^+ T cells from PBMCs of the gastric cancer patients and inhibitory effect on CD4^+ CD25^- T cells in vitro

作　　者：王凌云[1] 陈梅[2] 朱兆华[1] 彭辉[3] 吴长有[3] 

机构地区：[1]中山大学附属第二医院消化内科,广东广州510120 [2]中山大学附属第二医院检验科,广东广州510120 [3]中山大学基础医学院免疫教研室,广东广州510180

出　　处：《中国病理生理杂志》2006年第11期2202-2206,共5页Chinese Journal of Pathophysiology

摘　　要：目的:探讨胃癌患者外周血单个核细胞(PBMCs)中的CD4+CD25+T细胞体外增殖及对CD4+CD25-T细胞增殖的影响。方法:以免疫磁性分离方法(MACS)分选出胃癌患者外周血单个核细胞中的CD4+CD25+T及CD4+CD25-T细胞后,用流式细胞仪分析细胞的纯度及活力;再以小鼠抗人CD3单抗、小鼠抗人CD28单抗及rh IL-2作为共刺激因子,观察与CD4+CD25-T细胞共培养时,CD4+CD25+T细胞对CD4+CD25-T细胞增殖的抑制效应。结果:(1)分选后健康对照组及胃癌患者PBMC中CD4+CD25+T细胞纯度分别为83.8%±1.84%、84.13%±2.77%,两者相比,无显著差异(P>0.05);(2)经MACS分选后正常对照组与胃癌患者CD4+CD25+T细胞活力分别为98.52%±0.72%、97.80%±0.95%,两者相比,无显著差异(P>0.05);(3)无论是健康对照还是胃癌患者的CD4+CD25+T均具有明显抑制效应性T细胞如CD4+CD25-T细胞的增殖,随着CD4+CD25+T细胞数的增加,这种抑制增殖的能力也相应增加,当CD4+CD25+∶CD4+CD25-T达1∶1时,抑制率最大达到50%。结论:MACS分选法能够分选出高纯度及活力的CD4+CD25+T细胞,分选后CD4+CD25+T细胞在体外均能抑制CD4+CD25-T细胞增殖,且这种抑制效应呈一定效靶比关系。AIM ： To investigate the proliferation of CD4^＋ CD25^＋ T cells from PBMCs of the gastric cancer patients and the inhibitory effect on CD4^＋ CD25^- T cells in vitro. METHODS： Magnetic activated cell sorting （MACS） method was used to separate CD4^＋CD25^＋ T and CD4^＋ CD25^- T cells from peripheral blood monocytic lymphoeytes in the gastric cancer patients, and then the purity and activity of CD4^＋ CD25^＋ T cells were analyzed with flow eytometer. After stimulated with anti- CD3 Ab, anti- CD28 Ab and rh IL -2, CD4^＋CD25^- and CD4^＋CD25^＋ T cells were eocultured. The inhibitory effect of CD4^＋ CD25^＋ T on CD4^＋ CD25^- T cells was assayed by [^3 H ] thymidine proliferation experiment. RESULTS： （ 1 ） After sorting, CD4^＋ CD25^＋T cells purity in healthy control and gastric cancer patients were 83.80% ± 1.84% and 84. 13% ±2.77% , respectively. No significant difference between the two groups （P 〉0.05） was observed. （2） The activity of CD4^＋ CD25^＋ and CD4^＋ CD25^- T cells in healthy control and the gastric cancer patients after sorting were 98.52% ± 0. 72% and 97.80% ± 0. 95%. There was no significantly difference between the two groups （ P 〉 0.05 ）. （ 3 ） CD4^＋ CD25^＋T cells obviously inhibited the CD4^＋ CD25^- T cell proliferation in vitro. The inhibition achieved to maximum in eoeulture of CD4^＋ CD25^＋ T cells together with CD4^＋ CD25^- T cells （ ratio of 1 ： 1 ）. CONCLUSION ： The MACS system can effectively isolate CD4^＋ CD25^＋ and CD4^＋ CD25^- T cells. After sorting, CD4^＋CD25^＋ T cells obviously inhibit the proliferation of CD4^＋ CD25^-T cells in vitro and the inhibitory effect display an effect - target ratio relationship.

关 键 词：胃肿瘤 CD4^+CD25^+T细胞 CD4^+CD25^-T细胞 细胞增殖 

分 类 号：R363[医药卫生—病理学]