遗传基因组学(Genetical genomics)的研究进展  

Advance in Genetical Genomics

作　　者：刘秀艳[1] 谢正苗[1] 陈惠哲[2] 

机构地区：[1]杭州电子科技大学 [2]中国水稻研究所,杭州310006

出　　处：《生物化学与生物物理进展》2006年第11期1030-1034,共5页Progress In Biochemistry and Biophysics

基　　金：浙江大学环境修复与生态健康教育部重点实验室开放基金资助项目(050101);浙江省教育厅科研基金项目资助项目.~~

摘　　要：遗传基因组学(geneticalgenomics)是将微阵列技术和数量性状座位(QTL)分析结合起来,全基因组水平上定位基因表达的QTL(eQTL).它为研究复杂性状的分子机理和调控网络提供全新的手段.遗传基因组这个概念和研究策略在2001年由Janson和Nap首先提出,到目前为止,遗传基因组学已应用于酵母、老鼠、人以及玉米等植物.研究结果表明:基因表达水平的差异是可遗传的复杂性状;eQTL可以分为顺式作用eQTL和反式作用eQTL,顺式作用eQTL就是某个基因的eQTL定位到该基因所在的基因组区域,表明可能是该基因本身的差别引起mRNA水平的差别,反式作用就是eQTL定位到其他基因组区域,表明其他基因的差别控制该基因mRNA水平的差异.将eQTL结果、基因功能注解以及多种统计分析方法相结合,不仅能更准确地鉴别控制复杂性状及其相关基因表达的候选基因,而且能构建相应的基因调控网络.Genetical genomics is combining of microarry technology and quantitative trait loci （QTL） analysis, mapping expression QTL （eQTL） in global level. It provides a novel procedure for revealing the molecular mechanism and regulatory network of complex trait. The conception and strategy of genetical genomics were brought forward by Janson and Nap in 2001. By now, genetical genomics has been applied on yeast, mouse, human and plant such as maize. These results indicated that the variation of expression level of genes is heritable complex trait. Expression QTLs are classified into cis-acting and trans-acting. Cis-acting eQTL is located on the same genomic region of the regulated gene, meaning the variation of mRNA level could be due to the polymorphism in the gene itself. Trans-acting eQTL is located on other genomic region, meaning other gene controls the variation of mRNA level. The integration of eQTL results, gene function annotation and statistic analysis will not only more precisely identify candidate genes controlling complex traits and the expression of related genes, but also construct regulatory networks for these complex traits.

关 键 词：微阵列技术 基因表达的数量性状座位(QTL) 基因调控网络 

分 类 号：Q55[生物学—生物化学]