霉酚酸酯对局灶节段性肾小球硬化(FSGS)治疗作用的研究  

Effects of Mycophenolate Mofetil on Focal Segmental Glomerulosclerosis

作　　者：郑东辉[1] 邢昌赢[2] 陈菊花[1] 徐培敬[1] 任胜利[2] 

机构地区：[1]江苏省淮安市第二人民医院/徐州医学院附属淮安医院肾脏科,223002 [2]南京医科大学附属第一医院肾脏科

出　　处：《医学理论与实践》2006年第11期1251-1254,共4页The Journal of Medical Theory and Practice

基　　金：江苏省卫生厅135工程重点人才基金项目(135-034)

摘　　要：目的:用霉酚酸酯(MMF)对以阿霉素肾病大鼠建立的局灶节段性肾小球硬化(FSGS)模型,进行干预,检测结缔组织生长因子(CTGF)的表达,研究霉酚酸酯对FSGS的治疗作用,并探讨作用机理。方法:SD大鼠18只,分为对照组、阿霉素肾病组、MMF治疗组(每组大鼠6只)。肾病组、治疗组大鼠尾静脉一次性注入阿霉素7.5mg/kg,对照组大鼠尾静脉注入等量生理盐水。治疗组于第6周起MMF 20mg.kg-1.d-1混悬于1mL蒸馏水灌胃,其他组等量蒸馏水灌胃。第10周处死所有大鼠,观察肾组织病理变化,并以免疫组织化学、Western blot方法检测肾组织CTGF蛋白水平。结果:阿霉素肾病组大鼠较对照组大鼠肾小球系膜及基质明显增生,免疫组织化学染色及western blot显示肾小球和肾小管区CTGF蛋白表达明显上升(P<0.05),霉酚酸酯治疗组肾小球系膜和基质增生较肾病组明显减轻,肾小球和肾小管区CTGF蛋白表达明显低于肾病组(P<0.05)。结论:霉酚酸酯可以减轻肾脏间质纤维化病变,机理与抑制CTGF的表达有关。Objective： To detect the changes of connective tissue growth factor （CTGF） in adriamycin nephropathy rats, study the effects of mycophenolate mofetil on Focal segmental glomerulosclerosis, and investigate its possible mechanisms. Methods： 18 SD rats were randomly divided into three groups： control group （CG, n = 6）, FSGS model group （FG, n = 6）, MMF treated group （TG, n = 6）. Rats in MG and TG were given adriamycin 7.5mg/kg through vena caudalis. At the same time, an equal volume of normal saline was given to the rats in CG by the same method. The rats in TG received MMF 20mg·kg^-1·d^-1 by daily gastric garage from the 6th week. Renal pathological changes were evaluated at the 10th week. The expression of connective tissue growth factor（CTGF） were analyzed by immunohistochemistry and western blot. Results： The proliferation of the mesangial cells and the accumulation of the extracellular matrix in FG were more remarkable than that in CG. The expression of CTGF protein increased significantly （P〈0.05）. The proliferation of the TG rats were ameliorated, the expression of CTGF decreased markedly in TG rats （P〈0.05）. Conclusions： MMF can ameliorate kidney interstitial fibrosis, and the effect is related to decrease of CTGF expression.

关 键 词：霉酚酸酯(MMF) 阿霉素肾病(ADR) 足细胞(podocyte) 局灶节段性肾小球硬化(FSGS) 结缔组织生长因子(CTGF) 

分 类 号：R364.32[医药卫生—病理学]