人体胆石中的自由基信号初探  

FREE RADICAL SIGNAL IN HUMAN GALLSTONES

作　　者：沈韬[1] 刘文天[1] 傅贤波[1] 吴谨光 周孝思[1] 

机构地区：[1]北京医科大学第三医院普通外科,北京大学化学系

出　　处：《北京医科大学学报》1996年第5期361-362,370,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：探讨自由基对胆色素结石形成的影响。方法：用电子顺磁共振谱（ＥＰＲ）分析胆石，用ＦＴ－ＩＲ测定胆红素钙含量。结果：胆固醇结石的ＥＰＲ信号很弱或检测不出。胆色素结石则可同时具有自由基和过渡金属离子的ＥＰＲ特征，其中心谱线（ｇ＝２．００４０，线宽＝９．５Ｇ）由自由基引起，且强度与胆石中胆红素钙的含量正相关（ｒ＝＋０．９９５，Ｐ＜０．００１），结论：胆色素结石中存在源于胆红素类化合物的自由基。自由基对胆色素结五中胆红素聚合物的形成可能有重要影响。Objective:To investigate the role of free radical in the formation of pigment gallstones. Meth-ods:Gallstones were assayed by electron paramagnetic resonance(EPR)spectroscopy. The contents of calcium bilirubinate in gallstones were determined by FT-IR, Results:Very weak or no EPR signals were detected from cholesterol gallstones, and the features of free radical and transition metal ions were found in pigment gallstones. The central line(q=2.0040,line width=9. 5G) in EPR spectra of pigment gallstones is produced by free radical,and its intensities correlated linearly with the contents of calcium bilirubinate in pigment gallstones(r=+0.995,P<0.001).Conclusion :Free radical existed in pigment gallstones,and it arose from bilirubin compounds in gallstones. Free radical might play an important role in the formation of bilirtibin polymers in pigment gallstones.

关 键 词：胆结石 自由基 

分 类 号：R575.620.2[医药卫生—消化系统]