cyclin E、p16ink4、ki67在宫颈脱落细胞中的表达及其与HPV_(16/18)感染的相关性分析  

Relations Between the Experssion of cyclin E、p16ink4、ki67 and HPV_(16/18) Infection in Cervical Exfoliated Cells

作　　者：赵富玺[1] 郭俊成[2] 崔克[2] 熊思东[3] 

机构地区：[1]山西大同大学医学院病原生物与免疫学教研室 [2]山西大同大学医学院 [3]复旦大学上海医学院

出　　处：《大同医学专科学校学报》2006年第3期1-3,5,共4页Journal of Datong Medical College

基　　金：山西省自然科学基金资助(20051115)

摘　　要：目的:研究cyclinE、p16ink4、ki67在宫颈脱落细胞中的表达水平及其与HPV16/18感染的相关性,探讨其对宫颈癌高危人群筛查的意义。方法:采用免疫组织化学方法对78例宫颈脱落细胞标本进行cyclinE、p16ink4、ki67检测,同时应用多重引物PCR技术检测HPV16/18。结果:cyclin E、p16ink4、ki67在宫颈癌细胞中的表达水平较ASCUS差异均有统计学意义(P<0.005);各级宫颈癌细胞中HPV16的阳性率较ASCUS差异均有统计学意义(x2=25.27,P<0.005),随着宫颈上皮细胞的损伤程度加重而阳性率升高,差异也有统计学意义(P<0.01)。p16ink4和ki67在宫颈癌细胞中的表达水平与HPV16高度相关(rs=1.0,P<0.05);而cyclinE的表达与HPV16相关性较小(rs=0.4,P<0.05)。HPV18的阳性例数较少,在各项分析中差异均无统计学意义(x2=3.68,P>0.05)。结论:cyclinE、p16ink4及ki67在宫颈癌细胞中的高度表达与HPV16感染有关,它们均可能作为有价值的诊断指标应用于宫颈癌高危人群筛查,且cyclinE对宫颈癌的早期诊断意义更大。Objective： To confirm the relations between the expression of cyclin E, p16ink4, ki67 and HPV16/18 infection using cervical exfoliated cells, and evaluate the usefulness of cyclin E, p16ink4 and ki67 as biomarkers for screening of cervical carcionomas. Methods：The expression of cyclin E, p16ink4 oncoproteins and ki67 proliferative activity was evaluated immuohistochemically in 78 cervical exfoliated epithelial specimens. Human papillomavirus type 16 and 18 （HPV16/18） infection was assessed by polymerase chain reaction（PCR） using type specific primers. Results： Cyclin E, p16ink4 and ki67 were all overexpressed in cervical preneoplasia and neoplasia cells, compared with little expressed in ASCUS（ P 〈 0. 005）. Overexpression of cyclin E was observed in CIN Ⅰ （P 〈 0.01 ）, p16ink4 and ki67 overexpressed in invasive carcinoma（ 100 % and 90.9 % respectively）. The degree of p16ink4 and ki67 expression correlated well with the degree of cervical neopl＆sia （P〈0. 005）. HPV16 infection was assessed at all stages cervical neoplasia samples, and a significant relationship with the degree of cervical epithelial lession was observed at the same time. The expression level of p16ink4 and ki67 seemed to be more closely associated with HPV16 infection than cyclin E did（ rs = 1.0 vs rs = 0.4）. HPV18 was found positive in only 1 case in CINⅠ and 4 cases in CINⅡ-Ⅲ. Therefore no significance was found on statistical analysis （P〈 0. 005）. Conclusion： Cyclin E, p16ink4 and ki67 should be regarded as useful biomarkers of HPV-related cervical neoplasias, and be used for screening patients at high risk of developing cervical carcinomas. Moreover, cyclin E might be a significant cytologic marker for the primary screening of cervical carcinomas.

关 键 词：细胞周期蛋白E 蛋白质P16 宫颈上皮内瘤样病变 乳头状瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤]