银杏叶提取物对实验性肝纤维化逆转的影响  

Effect of Ginkgo Biloba Extract on CCl_4-induced Liver Fibrosis in Rats

作　　者：王莉[1] 李曙平[1] 蔡克银[1] 罗燕军[2] 

机构地区：[1]广州军区武汉总医院老年一科,武汉430070 [2]湖北省妇女儿童医院

出　　处：《华南国防医学杂志》2006年第5期18-21,共4页Military Medical Journal of South China

摘　　要：目的研究银杏叶提取物对大鼠肝纤维化逆转的影响及其可能的机制。方法应用CCl4诱导大鼠肝纤维化模型,造模完成后给予银杏叶提取物干预肝纤维化的逆转,应用H&E、Masson和Gordon-Sweet染色观察肝组织病理纤维化分级,生化法检测肝功能指标,RT-PCR法测定肝组织中基质金属蛋白酶-13(MMP-13)表达,免疫组化法检测肝组织中αSMA表达。结果给予银杏叶提取物可以促进肝纤维化的逆转(P<0.05),改善肝功能(P<0.05),增加恢复过程中肝组织MMP-13的表达(P<0.05),使αSMA阳性细胞数减少(P<0.05)。结论银杏叶提取物可促进CCl4肝纤维化的逆转,可能与银杏叶提取物增加肝组织中MMP-13表达、抑制肝脏星状细胞活化、促进活化星状细胞凋亡有关。Objective To explore the effect of Ginkgo biloba extract （GbE） on the development and reversion of liver fibrosis and its mechanism. Methods The model of CCI4-induced liver fibrosis was established in rats. The rats with liver fibrosis were treated with GbE. The liver fibrosis was graded by histopathological examinations （H＆E, Masson and Gordon-Sweet staining） and the expression of metalloproteinase-13 （ MMP-13 ） in the liver tissues was determined by RT-PCR. Blood samples were collected for determination of alanine aminotransferase （ALT） and aspartate aminotransferase （AST）. The expression of αSMA in liver tissues was detected by immunohistochemistry. Results Liver fibrosis and expression of αSMA in GbE group was significantly decreased compared to the control group （ P 〈0.05 ）. The level of ALT and AST were significantly decreased and the expression of MMP-13 significantly increased in GbE group compared to the control group（ P 〈0.01 ）. Conclusions GbE can relieve liver fibrosis induced by CCI4 in rats. Its mechanism is possibly related to GbE promoting the expression of MMP-13 and suppressing the activation and promoting the apoptosis of hepatic stellate cells （ HSC）.

关 键 词：银杏叶提取物 肝纤维化 四氯化碳 大鼠 组织金属蛋白酶-13 

分 类 号：R575.2[医药卫生—消化系统]