ROCK-Ⅰ蛋白活性变化对卵巢癌细胞恶性表型的影响  

Effect of the activity change of ROCK-Ⅰ on the malignant behaviors of ovarian tumor cells.

作　　者：韩志强[1] 洪振亚[2] 胡春霞[3] 胡轶[1] 陈彩虹[1] 卢运萍[1] 周剑峰[2] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科 [2]华中科技大学同济医学院附属同济医院血液科 [3]海南医学院附属医院妇产科

出　　处：《现代妇产科进展》2006年第10期735-738,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金项目(No:30371657);国家重点基础研究发展规划项目(No:2002CB51300)

摘　　要：目的:探讨ROCK-Ⅰ蛋白活性的改变对卵巢癌细胞恶性表型的影响。方法:将ROCK-Ⅰ的显性激活突变体p160△3以脂质体介导,转染人卵巢癌细胞系SW626、SKOV3、A2780和CaOV-3细胞,采用RT-PCR与Westernblot印迹法检测转染前后p160△3mRNA和蛋白的表达,Boyden小室观察该突变体对4种卵巢癌细胞株侵袭及迁移能力的影响,MTT比色法测定转染前后细胞增殖及粘附能力的变化。结果:转染后,ROCK-Ⅰ的显性激活突变体p160△3在细胞内获得有效表达。转染了p160△3的SW626、SK-OV3、A2780和CaOV-3细胞的侵袭能力较各自的对照组分别提高(31.1±6·4)%、(33.0±2.0)%、(24.5±8)%及(39.4±2.8)%;同时四株细胞的随机运动能力较各自的对照组分别提高(31.9±1.0)%、(31.5±1.9)%、(23.0±0.5)%及(38.7±1·2)%,定向运动能力较各自的对照组分别提高(33.9±1.1)%、(33.0±3.6)%;(25.0±3.9)%及(40·2±2.6)%。转染后两株细胞的体外粘附能力及增殖能力均未显示出明显变化。空质粒pCAG-myc转染组与对照组间差异无显著统计学意义(P>0.05)。结论:ROCK-Ⅰ蛋白活性的改变与人卵巢癌细胞的体外侵袭与迁移密切相关,ROCK-Ⅰ蛋白活性的提高明显促进卵巢癌肿瘤细胞的侵袭与迁移。Objective：To investigate the possible role of ROCK- I in ovarian cancer invasion and metastasis. Methods：The ROCK- I dominant active mutant p160A3 was transfected into SW626,SKOV3,A2780 and CaOV-3 cell lines mediated by LipofectamineTM 2000. The mRNA and protein of p160 △3 were detected by RT-PCR and Western-blot assay. Boyden chamber was used to assess the effect of p160 △3 on the invasion and migration of the cell lines. The changes in the adhesion and proliferation of the transfected cells were detected by MTT assay. Results： After transfection, p160 △3 was transcripted and translated effectively in SW626,SKOV3 ,A2780 and CaOV-3 cells. Compared with control group, the invasion capbility of transfected cells was increased 31.1% ± 6.4% ,33.0% ± 2.0% ,24.5% ± 8% and 39.4% ± 2.8% respectively. The random migratory activity of the four cell lines was enhanced 31.9% ± 1.0% ,31.5% ± 1.9% ,23.0% ±0.5% and 38.7% ± 1.2% respectively and the chemotaxis activity was promoted 33.9% ± 1.1% ,33.0% ±3.6% ,25.0% ±3.9% and 40. 2% ± 2.6%. No significant difference was found in the adhesion and proliferation of the cells between the transfectants with p160 △3 and the control. Conclusion：The activity of ROCK- I may play a crucial role in invasion and metastasis of ovarian cancer. The invasion and migration of ovarian cancer cells can be enhanced by the increased ROCK- I protein activity.

关 键 词：ROCK-I 显性激活突变体 转移 卵巢肿瘤 

分 类 号：R737.31[医药卫生—肿瘤]